School of Life Sciences, Faculty of Medicine and Health Sciences, University of Nottingham, Nottingham, UK.
Biology Department, Faculty of Science, University of Sulaimani, Sulaimani, Iraq.
J Med Microbiol. 2019 Apr;68(4):642-648. doi: 10.1099/jmm.0.000935. Epub 2019 Feb 12.
The liver-expressed pattern recognition receptors mannose-binding lectin (MBL), ficolin-2 and ficolin-3 contribute to the innate immune response by activating complement. Binding of soluble ficolin-2 to viral pathogens can directly neutralize virus entry. We observed that the human hepatoma cell line HuH7.5, which is routinely used for the study of hepatotropic viruses, is deficient in expression of MBL, ficolin-2 and ficolin-3. We generated a cell line that expressed and secreted ficolin-2. This cell line (HuH7.5 [FCN2]) was more resistant to infection with hepatitis C virus (HCV), ebolavirus and vesicular stomatitis virus, but surprisingly was more susceptible to infection with rabies virus. Cell-to-cell spread of HCV was also inhibited in ficolin-2 expressing cells. This illustrates that ficolin-2 expression in hepatocytes contributes to innate resistance to virus infection, but some viruses might utilize ficolin-2 to facilitate entry.
肝表达模式识别受体甘露糖结合凝集素（MBL）、ficolin-2 和 ficolin-3 通过激活补体有助于先天免疫反应。可溶性 ficolin-2 与病毒病原体的结合可以直接中和病毒进入。我们观察到，人肝癌细胞系 HuH7.5 常用于研究嗜肝病毒，其 MBL、ficolin-2 和 ficolin-3 的表达缺失。我们生成了一种表达和分泌 ficolin-2 的细胞系。该细胞系（HuH7.5 [FCN2]）对丙型肝炎病毒（HCV）、埃博拉病毒和水疱性口炎病毒的感染更具抵抗力，但令人惊讶的是，对狂犬病病毒的感染更敏感。HCV 的细胞间传播也在 ficolin-2 表达细胞中受到抑制。这表明肝细胞中 ficolin-2 的表达有助于先天抵抗病毒感染，但某些病毒可能利用 ficolin-2 来促进进入。
