不同年龄大鼠大脑皮质中胆碱能神经元的突触前和突触后标记物。

Pre- and postsynaptic markers of cholinergic neurons in the cerebral cortex of rats of different ages.

作者信息

Sirviö J, Hervonen A, Valjakka A, Riekkinen P J

机构信息

Department of Neurology, University of Kuopio, Finland.

出版信息

Exp Gerontol. 1988;23(6):473-9. doi: 10.1016/0531-5565(88)90059-9.

DOI:10.1016/0531-5565(88)90059-9

PMID:3074925

Abstract

In order to investigate the effect of aging on postsynaptic muscarinic binding, we measured 3H-PZ binding in the cerebral cortex of male Fisher 344 rats of different ages. ChAT activity was measured as a presynaptic marker of the cholinergic system. Total and salt-soluble AChE activities were also measured. Maximal binding of 3H-PZ and ChAT activity did not change during aging. The ratio of detergent-soluble AChE to salt-soluble AChE was lower in 30-month-old rats than in 4-month-old rats. The results of the present study do not indicate that the amount of M1 muscarinic receptors proposed to be located postsynaptically decreases during aging. In senescent rats, however, the proportions of salt-soluble and detergent-soluble AChE may differ from those in young rats.

摘要

为了研究衰老对突触后毒蕈碱结合的影响，我们测量了不同年龄雄性Fisher 344大鼠大脑皮层中3H-PZ的结合情况。测量了ChAT活性作为胆碱能系统的突触前标志物。还测量了总AChE活性和盐溶性AChE活性。衰老过程中3H-PZ的最大结合量和ChAT活性没有变化。30月龄大鼠中去污剂可溶性AChE与盐溶性AChE的比值低于4月龄大鼠。本研究结果并未表明突触后定位的M1毒蕈碱受体数量在衰老过程中减少。然而，在衰老大鼠中，盐溶性和去污剂可溶性AChE的比例可能与年轻大鼠不同。