免疫抑制剂对小鼠脾脏T和B淋巴细胞增殖反应的影响。I. 培养条件及评估系统。

Effect of immunosuppressive agents on the blastogenic response of mouse spleen T and B lymphocytes. I. Culture conditions and the evaluation system.

作者信息

Pospísil M, Dráber P, Hofman J, Nouza K, Klapácová J

出版信息

Folia Biol (Praha). 1978;24(3):185-98.

Abstract

A microplate culture system has been standardized for blastic transformation of lymphocytes, using various mitogens more or less specific to T lymphocytes (concanavalin A, phytohaemagglutinin, erythrogenic toxin) or B lymphocytes (lipopolysaccharides and their lipids A, pokeweed mitogen). The mitogen-stimulated lymphocyte activation was examined by the use of the nucleolar test determining the state of nucleolar RNA synthesis by morphologic criteria. In this introductory paper to a series of papers analyzing the specificity of action of the various chemical and biological immunosuppressive agents on the blastogenic responses of T and B lymphocytes, optimal concentrations of cells and blastogenic substances and other parameters were tested, and the kinetics of transformation was investigated in detail.

摘要

一种用于淋巴细胞增殖转化的微孔板培养系统已标准化，该系统使用了对T淋巴细胞（刀豆球蛋白A、植物血凝素、红细胞生成素）或B淋巴细胞（脂多糖及其脂质A、商陆有丝分裂原）或多或少具有特异性的各种促有丝分裂原。通过使用核仁试验，根据形态学标准确定核仁RNA合成状态，来检测促有丝分裂原刺激的淋巴细胞活化情况。在这篇介绍性论文中，作为一系列分析各种化学和生物免疫抑制剂对T和B淋巴细胞增殖反应作用特异性的论文的开篇，测试了细胞和促有丝分裂物质的最佳浓度以及其他参数，并详细研究了转化动力学。