Tsugawa H, Suzuki M
Aomori Green BioCenter, 221-10, Yamaguchi, Nogi, Aomori, 030-0142, Japan e-mail:
Plant Cell Rep. 2000 Mar;19(4):371-375. doi: 10.1007/s002990050742.
A method was developed to maintain plant regeneration activity of rice cells (Oryza sativa L.) using embryogenic callus. Calluses were cultured in suspension, then on solid medium, to form compact globular callus resistant to low-temperature stress and with high plant regeneration activity. Callus preserved at 5 °C for 5 months regenerated plants from protoplasts at a frequency higher than from non-preserved callus from cv. Nipponbare, and cv. Koshihikari, but at lower rates from cv. Akitakomachi. Similar results were obtained from protoplasts of the three cultivars. Callus preserved at 5 °C for 8 months incurred cell damage, yet some surviving cells divided in suspension culture and eventually regenerated whole plants. Preserved and non-preserved regenerated plants showed similar levels of somaclonal variation.
开发了一种利用胚性愈伤组织维持水稻细胞（Oryza sativa L.）植株再生活性的方法。将愈伤组织进行悬浮培养，然后在固体培养基上培养，以形成对低温胁迫具有抗性且具有高植株再生活性的紧密球形愈伤组织。在5°C下保存5个月的愈伤组织，其原生质体再生植株的频率高于来自日本晴和越光品种未保存愈伤组织的频率，但秋田小町品种的再生率较低。从这三个品种的原生质体中也获得了类似的结果。在5°C下保存8个月的愈伤组织出现细胞损伤，但一些存活细胞在悬浮培养中分裂，最终再生出完整植株。保存和未保存的再生植株表现出相似水平的体细胞克隆变异。
