甘薯贮藏根花青素产生细胞系的建立及其特性

Establishment and characteristics of an anthocyanin-producing cell line from sweet potato storage root.

作者信息

Konczak-Islam I, Yoshinaga M, Nakatani M, Terahara N, Yamakawa O

机构信息

Laboratory of Upland Crop Genetic Resources, Department of Upland Farming, Kyushu National Agricultural Experiment Station, 6644 Yokoichi, Miyakonojo, Miyazaki 885-0091, Japan e-mail:

Department of Food Science and Technology, College of Horticulture, Minami-Kyushu University, Takanabe, Miyazaki 884-0003, Japan, , , , , , JP.

出版信息

Plant Cell Rep. 2000 Apr;19(5):472-477. doi: 10.1007/s002990050758.

DOI:10.1007/s002990050758

PMID:30754885

Abstract

Anthocyanin pigments accumulated in a cell line derived from storage-root explants of sweet potato (Ipomoea batatas L.) cv 'Ayamurasaki'. Somatic pro-embryos were induced on the explants cultured on Murashige and Skoog medium supplemented with 1 mg/l 2,4-D. The pro-embryo structures produced callus when transferred to MS medium with 0.5 mg/l 2,4-D. A cell line was isolated from this callus which accumulated anthocyanin pigment. The color value of the pigment extracted after 27 days of culture in MS medium with 2 mg/l 2,4-D was 8.2, which was very close to that of a pigment extracted from roots, which was 8.9. Most of the pigments from the cell extract were hydrophilic and appeared on the ODS-column HPLC with a lower retention time than the main anthocyanins of the root tissues. The majority of the pigments were identical with the root anthocyanins. Cell line-specific anthocyanins were detected.

摘要

花青素色素在源自甘薯（Ipomoea batatas L.）品种‘绫紫’贮藏根外植体的细胞系中积累。在添加了1 mg/l 2,4 - D的Murashige和Skoog培养基上培养的外植体上诱导出体细胞原胚。当转移到含有0.5 mg/l 2,4 - D的MS培养基上时，原胚结构产生愈伤组织。从该愈伤组织中分离出一个积累花青素色素的细胞系。在含有2 mg/l 2,4 - D的MS培养基中培养27天后提取的色素颜色值为8.2，与从根中提取的色素颜色值8.9非常接近。细胞提取物中的大多数色素是亲水性的，在ODS柱HPLC上出现的保留时间比根组织的主要花青素短。大多数色素与根花青素相同。检测到细胞系特异性花青素。