Costa M G C, Nogueira F T S, Figueira M L, Otoni W C, Brommonschenkel S H, Cecon P R
Departamento de Biologia Vegetal e Núcleo de Biotecnologia Aplicada à Agropecuária (BIOAGRO), Universidade Federal de Viçosa, 36571-000, Viçosa, MG, Brazil e-mail:
Plant Cell Rep. 2000 Jan;19(3):327-332. doi: 10.1007/s002990050021.
Cotyledon explants of tomato (Lycopersicon esculentum Mill. cvs 'Santa Clara', 'Firme' mutant, 'IPA-5' and 'IPA-6') were excised from 8- to 10-day-old in vitro-grown seedlings. Four different shoot induction media supplemented with timentin (300 mg l) were screened. When cotyledon explants were cultured on MS-based medium with 1.0 mg l zeatin plus 0.1 mg l IAA and supplemented with timentin, higher regeneration frequencies and a greater number of elongated shoots were obtained. It was observed that timentin caused an increase in the morphogenesis of in vitro cotyledon explants of tomato cultivars. In two of three cultivars tested, rooting of shoots was positively influenced, both in the presence and absence of timentin in the rooting medium, among shoots regenerated from explants derived from timentin-supplemented medium. The results confirm those of a previous investigation on the beneficial effects of this class of antibiotics on tomato regeneration and, consequently, its reliability for use in the transformation of this species.
番茄(Lycopersicon esculentum Mill. 品种‘Santa Clara’、‘Firme’突变体、‘IPA - 5’和‘IPA - 6’)的子叶外植体取自8至10日龄的离体培养幼苗。筛选了四种添加替卡西林(300 mg/l)的不同芽诱导培养基。当子叶外植体在含有1.0 mg/l玉米素加0.1 mg/l吲哚乙酸的MS培养基上培养并添加替卡西林时,获得了更高的再生频率和更多伸长的芽。观察到替卡西林使番茄品种离体子叶外植体的形态发生增加。在测试的三个品种中的两个品种中,在生根培养基中添加或不添加替卡西林的情况下,从添加了替卡西林的培养基衍生的外植体再生的芽的生根均受到积极影响。结果证实了先前关于这类抗生素对番茄再生有益作用的研究结果,因此,其在该物种转化中的使用可靠性。