Department of Agricultural Biotechnology, Bidhan Chandra Krishi Viswavidyalaya, Mohanpur, Nadia, West Bengal, 741252, India.
KL College of Agriculture, Koneru Lakshmaiah Education Foundation, Vaddeswaram, Guntur, Andhra Pradesh, 522502, India.
Transgenic Res. 2023 Apr;32(1-2):33-52. doi: 10.1007/s11248-023-00338-w. Epub 2023 Feb 20.
Agrobacterium tumefaciens-mediated plant transformation has become routine work across the world to study gene function and the production of genetically modified plants. However, several issues hamper the transformation process in a profound way, both directly and indirectly. One of the major concerns is the overgrowth of Agrobacterium, which occasionally appears after the co-cultivation phase of the explant. This phenomenon is reported in several species and seems to spoil the whole transformation process. There are multiple approaches being employed to counter this unwanted growth of bacteria in a few plant species. In reality, once the overgrowth appears, it becomes nearly impossible to cure it. Hence, for the prevention of this phenomenon, numerous factors are regulated. These factors are: explant nature, A. tumefaciens strain, T-DNA vector, co-cultivation (time and condition), acetosyringone, washing medium, antibiotics (type, concentration, combination, incubation period), etc. In this article, we discuss these factors based on available reports. It can be of immense help in formulating viable strategies to control A. tumefaciens overgrowth.
农杆菌介导的植物转化已成为全球范围内研究基因功能和生产转基因植物的常规工作。然而，一些问题严重地阻碍了转化过程，既有直接的也有间接的。其中一个主要问题是农杆菌的过度生长，这种情况偶尔会在外植体的共培养阶段之后出现。这种现象在几个物种中都有报道，似乎破坏了整个转化过程。有多种方法被用于在少数几种植物物种中对抗这种不需要的细菌生长。实际上，一旦过度生长出现，就几乎不可能治愈它。因此，为了预防这种现象，需要调节许多因素。这些因素包括：外植体性质、农杆菌菌株、T-DNA 载体、共培养（时间和条件）、乙酰丁香酮、洗涤培养基、抗生素（类型、浓度、组合、孵育期）等。在本文中，我们根据现有报道讨论了这些因素。这对于制定可行的策略来控制农杆菌过度生长有很大的帮助。
