Balboni Fiamma, Burbui Stefania, Lippi Giuseppe
a Laboratory Service , Istituto Fiorentino Cura e Assistenza IFCA , Firenze , Italy.
b Section of Clinical Biochemistry , University of Verona , Verona , Italy.
Scand J Clin Lab Invest. 2018 Nov-Dec;78(7-8):546-550. doi: 10.1080/00365513.2018.1517221.
This study aimed to verify glucose stability within centrifuged serum and lithium-heparin tubes stored at room temperature (RT) or 4 °C. Sixty paired serum (plus gel separator), lithium-heparin (plus gel separator) and K-EDTA tubes were centrifuged within 30 min from collection. Thirty serum and lithium-heparin tubes were then stored at RT, whilst the other 30 serum and lithium-heparin tubes were kept at 4 °C. Complete cell blood count was performed in serum and plasma after centrifugation, as well as in K-EDTA paired whole blood tubes. Glucose was measured immediately after centrifugation and 3, 6, 24, 48, 72 and 96 h afterwards. Immeasurable blood cells values were found in serum, whilst residual leukocytes and platelets were present in lithium-heparin plasma. Regardless of storage conditions, glucose concentration decreased 3 h after centrifugation in lithium-heparin tubes, displaying uninterrupted reduction until 96 h. Mean decrease per hour was higher in plasma tubes stored at RT than at 4 °C. Performance specification was exceeded between 6 and 24 h of storage in most plasma tubes. Glucose concentration significantly decreased in serum tubes between 24 and 48 h, regardless of storage conditions. The mean glucose variation never exceeded performance specification throughout the study period. Mean glucose decrease per hour in plasma was not associated with blood cells counts before and after centrifugation, and was probably attributable to the presence of blood cells entrapped within the gel. Delayed glucose measurement in centrifuged serum tubes may be clinically viable up to 96 h, whilst it may be unadvisable in centrifuged lithium-heparin tubes.
本研究旨在验证在室温（RT）或4°C下储存的离心血清管和锂肝素管内葡萄糖的稳定性。60对血清（加凝胶分离剂）、锂肝素（加凝胶分离剂）和K-EDTA管在采集后30分钟内进行离心。然后将30支血清管和锂肝素管储存在室温下，而另外30支血清管和锂肝素管保存在4°C。离心后在血清和血浆中以及在K-EDTA配对的全血试管中进行全血细胞计数。离心后立即以及之后3、6、24、48、72和96小时测量葡萄糖。在血清中发现血细胞值不可测，而锂肝素血浆中存在残留白细胞和血小板。无论储存条件如何，锂肝素管离心后3小时葡萄糖浓度下降，直至96小时持续下降。室温下储存的血浆管每小时平均下降幅度高于4°C下储存的血浆管。大多数血浆管在储存6至24小时之间超过了性能规格。无论储存条件如何，血清管中葡萄糖浓度在24至48小时之间显著下降。在整个研究期间，平均葡萄糖变化从未超过性能规格。血浆中每小时葡萄糖平均下降与离心前后的血细胞计数无关，可能归因于凝胶中截留的血细胞的存在。离心血清管中延迟测量葡萄糖在临床上可行至96小时，而在离心锂肝素管中可能不可取。
