Section of Clinical Biochemistry, University Hospital of Verona, Verona, Italy.
Medical Direction, University Hospital of Verona, Verona, Italy.
Clin Chem Lab Med. 2018 Nov 27;56(12):2125-2131. doi: 10.1515/cclm-2018-0523.
Although it is known that glucose concentration exhibits a time-dependent decay in uncentrifuged serum and lithium-heparin blood tubes, no evidence exists on how this variation may depend on blood cell counts (CBC) and volumes.
Venous blood was drawn from 30 non fasting healthy volunteers into three serum and three lithium-heparin tubes. One serum and lithium-heparin tubes were centrifuged within 15 min after collection and glucose was measured with a hexokinase assay. The second and third serum and lithium-heparin tubes were maintained at room temperature for 1 and 2 h after the first tubes were centrifuged. These other tubes were then centrifuged and glucose was measured. CBC was performed in the first lithium-heparin tube, before centrifugation.
The mean decrease of glucose was higher in lithium-heparin plasma than in serum (0.33 vs. 0.24 mmol/L/h; p<0.001). Glucose concentration decreased by 7% and 5% per hour in lithium-heparin plasma and serum, respectively. In univariate analysis, the absolute decrease of glucose concentration was associated with sex (higher in men than in women), red blood cell (RBC) count, hematocrit, white blood cell (WBC) count, neutrophils and monocytes in both lithium-heparin plasma and serum. In multivariate analysis, the decrease of glucose concentration remained independently associated with RBC, WBC, neutrophils and monocytes in both sample matrices. No significant association was found with platelet number and erythrocyte or platelet volume.
Glucose concentration decrease in uncentrifuged lithium-heparin and serum tubes depends on the baseline number of RBC, WBC, neutrophils and monocytes within the tubes.
虽然已知未离心的血清和锂肝素血液管中的葡萄糖浓度随时间呈下降趋势，但尚无证据表明这种变化如何取决于血细胞计数（CBC）和体积。
从 30 名非空腹健康志愿者中抽取静脉血，分别放入 3 支血清管和 3 支锂肝素管中。采集后 15 分钟内对其中一支血清管和锂肝素管进行离心，用己糖激酶法测定葡萄糖。将第二支和第三支血清和锂肝素管在第一支离心管离心后 1 和 2 小时保持在室温下。然后对这些其他管进行离心并测量葡萄糖。在第一支锂肝素管离心前进行 CBC。
锂肝素血浆中的葡萄糖平均下降幅度高于血清（0.33 与 0.24 mmol/L/h；p<0.001）。锂肝素血浆和血清中的葡萄糖浓度每小时分别下降 7%和 5%。在单变量分析中，葡萄糖浓度的绝对下降与性别（男性高于女性）、红细胞（RBC）计数、血细胞比容、白细胞（WBC）计数、中性粒细胞和单核细胞在锂肝素血浆和血清中均相关。在多变量分析中，在两种样本基质中，葡萄糖浓度的下降仍与 RBC、WBC、中性粒细胞和单核细胞独立相关。与血小板数以及红细胞或血小板体积无显著相关性。
未离心的锂肝素和血清管中的葡萄糖浓度下降取决于管内 RBC、WBC、中性粒细胞和单核细胞的基线数量。
