通过斑点酶联免疫吸附测定法检测麻风病抗原尿。
Detection of leprosy antigenuria through DOT-ELISA.
作者信息
Singh N B, Choudhary A
机构信息
Division of Microbiology, Central Drug Research Institute, Lucknow.
出版信息
Indian J Lepr. 1988 Oct;60(4):526-9.
Fifty-five samples of urine from different grades of leprosy patients and normal persons were processed for detection of PGL-1 antigen through DOT-ELISA on nitrocellulose paper strips using anti-human IgG horse raddish peroxidaseconjugate. About 66.6% of the paucibacillary and 100% of the multibacillary leprosy cases were detectable through this technique on the basis of differential colour development on the strips. Possibility of its use in the field conditions has been discussed.
采用抗人IgG辣根过氧化物酶结合物，通过硝酸纤维素纸条上的斑点酶联免疫吸附测定法（DOT-ELISA），对55份来自不同等级麻风病患者和正常人的尿液样本进行处理，以检测酚糖脂-1（PGL-1）抗原。基于纸条上颜色变化差异，通过该技术可检测出约66.6%的少菌型和100%的多菌型麻风病病例。文中还讨论了该技术在现场条件下使用的可能性。
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