Gupte M D, Raj C A, Kannan S, Desikan K V
CJIL Field Unit for Epidemiology of Leprosy, Avadi, Madras.
Indian J Lepr. 1988 Oct;60(4):566-71.
Skin smear direct microscopy is an important tool for diagnosis of leprosy. The study was planned to understand the reproducibility of skin smear reading by a trained technician. Skin smears were collected from known patients of leprosy from the field area. They were stained for acid fast bacilli following the standard cold staining procedure and were read following the Ridley scale. A sample of smears was re-examined on two occasions by the same technician, following blind procedure. There was a systematic under reading on the second occasion, which was attributed to the defective storage of the slides. However, the agreement between second and third examinations was very good (Concordance 81.34%, Kappa 0.74). The finding was confirmed on a repeat examination. It can be concluded that the Direct Skin Smear Microscopy is a reliable and reproducible technique under experimental conditions.
皮肤涂片直接显微镜检查是诊断麻风病的一项重要工具。本研究旨在了解经过培训的技术人员对皮肤涂片判读的可重复性。从疫区已知的麻风病患者处采集皮肤涂片。按照标准冷染色程序对涂片进行抗酸杆菌染色，并根据里德利量表进行判读。同一位技术人员按照盲法程序对一组涂片样本进行了两次复检。第二次复检存在系统性的判读不足，这归因于玻片保存不当。然而，第二次和第三次检查之间的一致性非常好（一致性81.34%，卡帕值0.74）。重复检查证实了这一结果。可以得出结论，在实验条件下，直接皮肤涂片显微镜检查是一项可靠且可重复的技术。
