Department of Chemistry, Faculty of Sciences, Tarbiat Modares University, Tehran, Iran.
Materials and Energy Research Center, Department of Semiconductors, Karaj, Iran.
J Sep Sci. 2019 Apr;42(8):1620-1628. doi: 10.1002/jssc.201801152. Epub 2019 Feb 27.
In the present study, a supramolecular solvent was formed from reverse micelle aggregates of octanol. The proposed supramolecular solvent was used for rapid extraction of some antidepressants drugs including amitriptyline, imipramine, desipramine, maprotiline, sertraline, and doxepin from biological samples. Alkanol-based supramolecular solvents have a unique array of physicochemical properties, making them a very attractive alternative to replace organic solvents in analytical extractions. The parameters affecting the extraction of target analytes (i.e., the volume of tetrahydrofuran and octanol as the major components comprising the supramolecular solvent, chain length of alkanols, sample solution pH, salt addition, and ultrasonic time) were investigated and optimized by factor by factor optimization method. Under the optimum conditions, preconcentration factors of 470, 490, 460, 385, 370, and 430 were obtained for amitriptyline, doxepin, imipramine, desipramine, maprotiline, and sertraline, respectively. The linear ranges and coefficients of determination (R ) were obtained in the range of 0.01-100 μg/L and 0.9974-0.9991, respectively. Also the limits of detection (S/N = 3) of 0.003-0.03 μg/L, and precisions (n = 5) of 4.9-8.9% were calculated. Finally, the method was successfully applied for the extraction of antidepressant drugs in biological samples, and relative recoveries in the range of 91-102% were obtained.
在本研究中，通过辛醇的反胶束聚集体形成了超分子溶剂。所提出的超分子溶剂用于快速提取一些抗抑郁药药物，包括阿米替林、丙咪嗪、去甲丙咪嗪、马普替林、舍曲林和多塞平从生物样本中。基于醇的超分子溶剂具有独特的理化性质，使其成为分析萃取中替代有机溶剂的极具吸引力的选择。通过单因素优化法研究并优化了影响目标分析物萃取的参数（即四氢呋喃和辛醇的体积作为构成超分子溶剂的主要成分、醇的链长、样品溶液 pH 值、加盐和超声时间）。在最佳条件下，分别获得了阿米替林、多塞平、丙咪嗪、去甲丙咪嗪、马普替林和舍曲林的预浓缩因子为 470、490、460、385、370 和 430。线性范围和相关系数（R）分别为 0.01-100μg/L 和 0.9974-0.9991。还计算了检出限（S/N=3）为 0.003-0.03μg/L，精密度（n=5）为 4.9-8.9%。最后，该方法成功应用于生物样品中抗抑郁药的提取，获得了 91-102%的相对回收率。
