Velázquez-Cervantes M A, Martínez-Castillo M, González-García L D, Vargas-Pavía T A, Martínez-Salazar M G, Mancilla-Herrera I, León-Reyes G, García-Cordero J, Helguera-Repetto A C, León-Juárez M
Departamento de Inmunobioquímica, Instituto Nacional de Perinatología Isidro Espinosa de los Reyes, Montes Urales 800, Col. Lomas Virreyes, CP 11000, Mexico City, Mexico.
Departamento de Infectología e Inmunología, Instituto Nacional de Perinatología Isidro Espinosa de los Reyes, Montes Urales 800, Col. Lomas Virreyes, CP 11000, Mexico City, Mexico.
Virus Genes. 2019 Jun;55(3):406-410. doi: 10.1007/s11262-019-01646-2. Epub 2019 Feb 13.
The respiratory syncytial virus (RSV) is the main pathogen associated with upper respiratory tract infections during early childhood. Vertical transmission of this virus has been suggested in humans, based on observations recorded during animal studies that revealed an association of RSV with persistent structural and functional changes in the developing lungs of the offspring. However, human placentas have not yet been evaluated for susceptibility to RSV infection. In this study, we examined the capacity of RSV to infect a human trophoblast model, the BeWo cell line. Our results suggest that BeWo cells are susceptible to RSV infection since they allow RNA viral replication, viral protein translation, leading to the production of infectious RSV particles. In this report, we demonstrate that a human placenta model system, consisting of BeWo cells, is permissive to RSV infection. Thus, the BeWo cell line may represent a useful model for studies that aim to characterize the events of a possible RSV infection at the human maternal-fetal interface.
呼吸道合胞病毒（RSV）是幼儿期上呼吸道感染的主要病原体。基于动物研究中记录的观察结果，即RSV与后代发育中肺部的持续结构和功能变化有关联，已有人提出该病毒可在人类中垂直传播。然而，尚未评估人胎盘对RSV感染的易感性。在本研究中，我们检测了RSV感染人滋养层细胞模型BeWo细胞系的能力。我们的结果表明，BeWo细胞易受RSV感染，因为它们允许RNA病毒复制、病毒蛋白翻译，从而产生有传染性的RSV颗粒。在本报告中，我们证明了由BeWo细胞组成的人胎盘模型系统对RSV感染具有易感性。因此，BeWo细胞系可能是一个有用的模型，用于旨在表征人类母胎界面可能发生的RSV感染事件的研究。
