肌肉抽搐过程的时间分辨X射线衍射研究。
A time-resolved X-ray diffraction study of muscle during twitch.
作者信息
Matsubara I, Yagi N
出版信息
J Physiol. 1978 May;278:297-307. doi: 10.1113/jphysiol.1978.sp012305.
Abstract
- A position sensitive X-ray counter was connected to a data-collection system which registered the outputs of the counter as a function of time. This enabled us to study time-dependent changes in the intensities of the equatorial reflexions from frog sartorius muscle during an isometric twitch. 2. The intensity ratio of the 1.0 and 1.1 equatorial reflexions (I1,0/I1,1) decreased during a twitch. The time at which the intensity ratio started to decrease coinsided approximately with the onset of tension development. No detectable change in the intensity ratio occurred during the latency relaxation. 3. The intensity ratio reached a minimum level approximately 90 msec before the peak of tension, and stayed at that level until the peak tension. 4. The intensity ratio returned to the 'resting' value 0.7--1.1 sec after the twitch tension had fallen to zero. 5. The observed decrease in the intensity ratio was interpreted as being caused by radial movements of the myosin projections from the vicinity of thick filaments to that of thin filaments. This leads us to conclude that, early in the rising phase of twitch tension, the number of myosin projections in the vicinity of thin filaments reaches the value obtained during a maximum isometric tetanus.
摘要
- 一个位置敏感型X射线计数器与一个数据采集系统相连，该系统将计数器的输出记录为时间的函数。这使我们能够研究青蛙缝匠肌等长收缩时赤道反射强度随时间的变化。2. 1.0和1.1赤道反射的强度比（I1,0/I1,1）在收缩过程中降低。强度比开始降低的时间大约与张力发展的开始时间一致。在潜伏期松弛期间，强度比没有可检测到的变化。3. 强度比在张力峰值前约90毫秒达到最低水平，并保持在该水平直到峰值张力。4. 在抽搐张力降至零后0.7 - 1.1秒，强度比恢复到“静息”值。5. 观察到的强度比降低被解释为是由于肌球蛋白突起从粗丝附近向细丝附近的径向运动引起的。这使我们得出结论，在抽搐张力上升阶段的早期，细丝附近的肌球蛋白突起数量达到最大等长强直收缩时获得的值。
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