Schoenberg M, Wells J B
Biophys J. 1984 Feb;45(2):389-97. doi: 10.1016/S0006-3495(84)84163-6.
The time course of force and stiffness during a twitch was determined at 6 and 26 degrees C in frog semitendinosus muscle bundles using the transmission time technique of Schoenberg, M., J.B. Wells, and R.J. Podolsky, 1974, J. Gen. Physiol. 64:623-642. Sarcomere shortening due to series compliance was also measured using a laser light diffraction technique. Following stimulation, stiffness developed more rapidly than force, but had a slower time course than published Ca2+ transients determined from light signals using Ca2+ sensitive dyes (Baylor, S.M., W.K. Chandler, and M.W. Marshall, 1982, J. Physiol. (Lond.). 331:139-177). Stiffness (S) did not reach its tetanic value during a twitch at 6 or 26 degrees C, although at 6 degrees C, it approached close to this value with S-twitch/S-tetanus = 0.82 +/- 0.07 (+/- SEM). During relaxation, force fell more rapidly than stiffness both for a twitch and also a tetanus. Also in this paper, several of the assumptions inherent in using the transmission time technique for the measurement of stiffness are considered in detail.
利用舍恩伯格、J.B. 韦尔斯和R.J. 波多尔斯基在1974年《普通生理学杂志》第64卷第623 - 642页中介绍的传播时间技术，测定了青蛙半腱肌束在6摄氏度和26摄氏度下一次单收缩过程中力和刚度随时间的变化过程。还使用激光衍射技术测量了由于串联弹性导致的肌节缩短。刺激后，刚度比力发展得更快，但与使用钙敏染料根据光信号测定的已发表的钙离子瞬变相比，其时间变化过程较慢（贝勒、S.M.、W.K. 钱德勒和M.W. 马歇尔，1982年，《生理学杂志》（伦敦），第331卷，第139 - 177页）。在6摄氏度或26摄氏度下的一次单收缩过程中，刚度（S）未达到其强直值，尽管在6摄氏度时，它接近该值，S单收缩/S强直 = 0.82±0.07（±标准误）。在松弛过程中，无论是单收缩还是强直收缩，力的下降都比刚度快。此外，在本文中，还详细考虑了使用传播时间技术测量刚度时所固有的几个假设。
