Tchorbadjieva M, Odjakova M K
Sofia University, Department of Biochemistry, Faculty of Biology, Dr. Zankov str. 8, 1421 Sofia, Bulgaria e-mail:
Plant Cell Rep. 2001 Jan;20(1):28-33. doi: 10.1007/s002990000284.
The isoenzyme pattern of esterases (EC 3.1.1.2) secreted into the medium of orchardgrass (Dactylis glomerata L.) embryogenic suspension cultures during defined stages of somatic embryogenesis was compared with that of non-embryogenic suspension cultures during unorganised cell proliferation. Isoelectric focusing revealed the presence of 7-14 predominantly acidic isoforms. Comparison with the corresponding cell-wall isoenzyme pattern showed minor, mainly quantitative differences. The pattern of intracellular soluble esterases did not change markedly during somatic embryo development. A unique esterase whose migration in two-dimensional gel electrophoresis corresponds to an apparent molecular mass of 36 kDa and pI=3.8 was detected only in embryogenic cultures at very early stages of development. Since this isoform appeared long before morphological changes had taken place, it could possibly be used as a biochemical marker for embryogenic potential in D. glomerata L. suspension cultures.
在体细胞胚胎发生的特定阶段，将果园草（鸭茅）胚性悬浮培养物培养基中分泌的酯酶（EC 3.1.1.2）同工酶模式，与非胚性悬浮培养物在无组织细胞增殖期间的同工酶模式进行了比较。等电聚焦显示存在7 - 14种主要为酸性的同工型。与相应的细胞壁同工酶模式比较显示出微小的差异，主要是数量上的差异。在体细胞胚胎发育过程中，细胞内可溶性酯酶的模式没有明显变化。仅在发育非常早期的胚性培养物中检测到一种独特的酯酶，其在二维凝胶电泳中的迁移对应于表观分子量36 kDa和pI = 3.8。由于这种同工型在形态变化发生之前很久就出现了，它可能用作鸭茅悬浮培养物中胚性潜力的生化标记。
