Dittoe Dana K, Atchley Julie A, Feye Kristina M, Lee Jung Ae, Knueven Carl J, Ricke Steven C
Department of Food Science and Center for Food Safety, University of Arkansas, Fayetteville, AR, United States.
Agricultural Statistics Laboratory, University of Arkansas, Fayetteville, AR, United States.
Front Vet Sci. 2019 Jan 30;6:6. doi: 10.3389/fvets.2019.00006. eCollection 2019.
The presence of spp. on poultry products is one of the leading causes of foodborne illness in the United States. Therefore, novel antimicrobial substances are being explored as potential interventions in poultry processing facilities. The objective of the current study was to evaluate the efficacy of varying concentrations of sodium bisulfate salt, SBS, alone or in combination with peracetic acid, PAA, in 15 s whole part dips. Ninety six drumsticks (4 replications, 8 treatments, 3 days) were inoculated separately in a 400 mL solution of nalidixic resistant (NA) Enteritidis (10 CFU/mL) and allowed to adhere for 60 to 90 min at 4°C for a final concentration of 10 CFU/g. The experimental treatments included: a no treatment (control), and 15 s dips in 300 mL of tap water alone (TW) or with the addition of 1; 2; and 3% SBS; 1; 2; and 3% SBS+PAA. After treatment, drumsticks were stored at 4°C until microbial sampling was conducted. On d 0, l, and 3, drumsticks were rinsed in 150 mL of nBPW for 1 min, 100 μL of rinsate was serially diluted, spread plated on XLT4+NA (20 μg/mL), and incubated aerobically at 37°C for 24 h. Log-transformed counts were analyzed using a randomized complete block design (day) using One-Way ANOVA, polynomial contrasts, and pairwise comparisons with means being separated by Tukey's HSD with a significance level of ≤ 0.05. A treatment by day interaction ( = 0.14071) was not substantial. Thus, the treatment effect was investigated separately by days. Over time, a linear trend was observed in . Enteritidis concentration when SBS was increased (1 < 2 < 3%). The concentration of . Enteritidis was different between 1% SBS and 1% SBS+PAA on d 0. However, the level of . Enteritidis was not different among drumsticks treated in 2 and 3% SBS and 2 and 3% SBS+PAA across d 0, 1, 3. The application of 3% SBS alone or in combination with 200 ppm of PAA is capable of reducing the presence of over a 3-d refrigeration period; potentially increasing the safety of poultry products for consumers.
在美国,家禽产品中存在某些特定菌种是食源性疾病的主要原因之一。因此,新型抗菌物质正在被探索作为家禽加工设施中的潜在干预措施。本研究的目的是评估不同浓度的硫酸氢钠(SBS)单独使用或与过氧乙酸(PAA)联合使用,在15秒的整只浸泡处理中的效果。九十六个鸡腿(4次重复,8种处理,3天)分别接种于400毫升对萘啶酸耐药(NA)的肠炎沙门氏菌(10 CFU/mL)溶液中,并在4°C下静置60至90分钟,以使最终浓度达到10 CFU/g。实验处理包括:不处理(对照),以及在300毫升自来水中单独浸泡15秒(TW)或添加1%、2%和3%的SBS;1%、2%和3%的SBS + PAA。处理后,鸡腿在4°C下储存,直至进行微生物采样。在第0天、第1天和第3天,将鸡腿在150毫升非缓冲蛋白胨水中冲洗1分钟,取100微升冲洗液进行系列稀释,并涂布于XLT4 + NA(20微克/毫升)平板上,在37°C有氧条件下培养24小时。使用随机完全区组设计(天数),通过单因素方差分析、多项式对比以及采用Tukey's HSD法进行均值的两两比较(显著性水平≤0.05),对对数转换后的计数进行分析。处理与天数的交互作用(P = 0.14071)不显著。因此,分别按天数研究处理效果。随着时间推移,当SBS浓度增加时(1% < 2% < 3%),肠炎沙门氏菌浓度呈现线性趋势。在第0天,1% SBS和1% SBS + PAA处理的鸡腿中肠炎沙门氏菌浓度不同。然而,在第0天、第1天和第3天,用2%和3% SBS以及2%和3% SBS + PAA处理的鸡腿中肠炎沙门氏菌水平没有差异。单独应用3% SBS或与200 ppm的PAA联合使用,能够在3天的冷藏期内降低肠炎沙门氏菌的存在;这有可能提高家禽产品对消费者的安全性。