Page S, Odds F C
Department of Microbiology, University of Leicester, UK.
J Gen Microbiol. 1988 Oct;134(10):2693-702. doi: 10.1099/00221287-134-10-2693.
The ability of purified human albumin, fibrinogen and transferrin to bind to Candida species was measured by immunofluorescence. The proteins all bound with high avidity to germ-tubes formed by Candida albicans, but did not bind to blastospores of C. albicans or other pathogenic Candida species, not even to parent blastospores bearing germ-tubes. The extent of binding of the proteins to C. albicans germ-tubes varied between growth media and from germ-tube to germ-tube. Strains of C. albicans that did not form germ-tubes were incapable of binding any of the proteins. There was evidence that purified fibrinogen bound to germ-tubes with higher avidity than albumin and transferrin. When germ-tubes were treated with whole human plasma or serum, indirect immunofluorescence revealed that proteins were bound all over the surface of C. albicans blastospore-germ-tube units, indicating behaviour different from that seen with the purified proteins tested alone or in mixtures. C. albicans cells grown in the presence of azole antifungal agents bound purified plasma proteins in the same way as cells untreated with the drugs. The results of this study suggest that binding of host proteins to the surface of C. albicans may not be a property related directly to virulence of the fungus in vivo.
通过免疫荧光法测定纯化的人白蛋白、纤维蛋白原和转铁蛋白与念珠菌属的结合能力。这些蛋白质都以高亲和力与白色念珠菌形成的芽管结合，但不与白色念珠菌的芽生孢子或其他致病性念珠菌属的芽生孢子结合，甚至不与带有芽管的亲本芽生孢子结合。这些蛋白质与白色念珠菌芽管的结合程度在不同生长培养基之间以及不同芽管之间有所不同。不形成芽管的白色念珠菌菌株无法结合任何一种蛋白质。有证据表明，纯化的纤维蛋白原比白蛋白和转铁蛋白以更高的亲和力与芽管结合。当用全人血浆或血清处理芽管时，间接免疫荧光显示蛋白质结合在白色念珠菌芽生孢子 - 芽管单元的整个表面，这表明其行为与单独测试或混合测试的纯化蛋白质不同。在唑类抗真菌剂存在下生长的白色念珠菌细胞与未用药物处理的细胞一样以相同方式结合纯化的血浆蛋白。这项研究的结果表明，宿主蛋白与白色念珠菌表面的结合可能不是一种与该真菌在体内毒力直接相关的特性。
