Martin M V
J Med Microbiol. 1979 May;12(2):187-93. doi: 10.1099/00222615-12-2-187.
Candida species isolated from the mouths of healthy children and of patients with denture stomatitis included strains of Candida tropicalis that formed germ tubes when incubated in serum. Twenty-six germ-tube-forming strains of C. albicans and of C. tropicalis were subcultured weekly for 9 wk on blood agar and on Sabouraud's agar and the ability of each subculture to form germ tubes was measured. All the strains of C. albicans formed almost as many germ tubes after nine weekly subcultures as they did when first isolated. By contrast, although all 26 strains of C. tropicalis formed germ tubes when first isolated, all had lost the ability to do so after six serial weekly subcultures. Germ-tube formation should not be the sole criterion for the identification of oral C. albicans strains.
从健康儿童口腔以及义齿性口炎患者口腔中分离出的念珠菌属包括热带念珠菌菌株，这些菌株在血清中孵育时会形成芽管。26株能形成芽管的白色念珠菌和热带念珠菌菌株在血琼脂和沙保弱琼脂上每周传代培养9周，并测定每次传代培养物形成芽管的能力。所有白色念珠菌菌株在每周传代培养9次后形成的芽管数量几乎与首次分离时一样多。相比之下，虽然所有26株热带念珠菌在首次分离时都能形成芽管，但在连续每周传代培养6次后，所有菌株都失去了这种能力。芽管形成不应作为鉴定口腔白色念珠菌菌株的唯一标准。
