Pilkington B J, Rose A H
Zymology Laboratory, School of Biological Sciences, Bath University, Bath, Avon, UK.
J Gen Microbiol. 1988 Oct;134(10):2823-30. doi: 10.1099/00221287-134-10-2823.
Sulphite inhibited growth of all four yeasts studied, Zygosaccharomyces bailii NCYC 563 being most sensitive and Saccharomyces cerevisiae NCYC 431 the least. Vertical Woolf-Eadie plots were obtained for initial velocities of 35S accumulation by all four yeasts suspended in high concentrations of sulphite. Equilibrium levels of 35S accumulation were reached somewhat faster with strains of S. cerevisiae than with those of Z. bailii. With all four yeasts, the greater the extent of 35S accumulation, the larger was the decline in internal pH value. Growth of S. cerevisiae TC8 and Z. bailii NCYC 563, but to a lesser extent of S. cerevisiae NCYC 431 and Z. bailii NCYC 1427, was inhibited when mid exponential-phase cultures were supplemented with 1.0 or 2.0 mM-sulphite, the decrease in growth being accompanied by a decline in ethanol production. Unless growth was completely inhibited, the sulphite-induced decline in growth was accompanied by production of acetaldehyde and additional glycerol.
亚硫酸盐抑制了所研究的所有四种酵母的生长，拜耳接合酵母NCYC 563最为敏感，酿酒酵母NCYC 431最不敏感。对悬浮于高浓度亚硫酸盐中的所有四种酵母的35S积累初始速度绘制了垂直的伍尔夫-伊迪曲线。酿酒酵母菌株达到35S积累平衡水平的速度比拜耳接合酵母菌株略快。对于所有四种酵母，35S积累的程度越大，内部pH值下降幅度越大。当对数中期培养物补充1.0或2.0 mM亚硫酸盐时，酿酒酵母TC8和拜耳接合酵母NCYC 563的生长受到抑制，但酿酒酵母NCYC 431和拜耳接合酵母NCYC 1427的生长受到的抑制程度较小，生长的下降伴随着乙醇产量的下降。除非生长完全受到抑制，亚硫酸盐诱导的生长下降伴随着乙醛和额外甘油的产生。
