Department of Pathology and Laboratory Medicine, Shin Kong Wu Ho-Su Memorial Hospital, Taipei, Taiwan.
School of Medical Laboratory Science and Biotechnology, Taipei Medical University, Taipei, Taiwan.
Environ Toxicol. 2019 May;34(5):659-665. doi: 10.1002/tox.22732. Epub 2019 Feb 13.
Ouabain, a cardiotonic steroid, was used for the treatment of heart failure and atrial fibrillation and induces cancer cell apoptosis in many human cancer cells including human leukemia cells. However, there are no reports to show the effects on immune responses in a leukemia mouse model. In this study, WEHI-3 cell generated leukemia mice were developed and treated by oral ouabain at 0, 0.75, 1.5, and 3 mg/kg for 15 days. Results indicated that ouabain did not affect body appearance, but decreased liver and spleen weights, B- and T-cell proliferation at all three doses treatment and increased CD19 cells at 3.0 mg/kg treatment, decreased CD3, CD11b, and Mac-3 cells levels compared with positive control. Furthermore, ouabain increased the macrophage phagocytosis from peripheral blood mononuclear cell and peritoneal cavity at all three doses treatment and increased NK cell activities. Ouabain restored GOT, GPT and LDH levels in WEHI-3 leukemia mice in vivo.
哇巴因，一种强心甾类化合物，曾被用于心力衰竭和心房颤动的治疗，并能诱导包括人白血病细胞在内的多种人类癌细胞凋亡。然而，目前尚无研究报道哇巴因对白血病小鼠模型免疫反应的影响。在本研究中，我们构建了 WEHI-3 细胞诱导的白血病小鼠模型，并通过灌胃给予 0、0.75、1.5 和 3 mg/kg 哇巴因治疗 15 天。结果表明，哇巴因治疗不影响动物外观，但可降低肝脾重量，抑制 B 和 T 细胞增殖，并在 3.0 mg/kg 剂量下增加 CD19 细胞，降低 CD3、CD11b 和 Mac-3 细胞水平。此外，哇巴因可增强外周血单个核细胞和腹腔巨噬细胞的吞噬作用，增加 NK 细胞活性。体内实验结果表明，哇巴因可恢复 WEHI-3 白血病小鼠的 GOT、GPT 和 LDH 水平。
