Coetzee J N, Bradley D E, du Toit L, Hedges R W
Department of Microbiology, University of Pretoria, South Africa.
J Gen Microbiol. 1988 Sep;134(9):2535-41. doi: 10.1099/00221287-134-9-2535.
Phage X-2, a filamentous rod about 950 nm in length, was isolated from sewage as plating on strains of Escherichia coli, Salmonella typhimurium or Serratia marcescens carrying either the IncX plasmid R6K, or the unique plasmid R775. Phage X-2 differs morphologically from a previously described very broad host range filamentous phage X which also lyses plasmid R6K-carrying strains and the phages differ in their resistance to inactivation by diethyl ether. Phage X-2 is serologically unrelated to phage X and the X-like phages IKe and I2-2. The adsorption site of the phage on the plasmid-bearing strains could not be determined but evidence implicating conjugative pili is presented.
噬菌体X-2是一种长度约为950纳米的丝状杆状噬菌体,它是从污水中分离出来的,通过接种在携带IncX质粒R6K或独特质粒R775的大肠杆菌、鼠伤寒沙门氏菌或粘质沙雷氏菌菌株上而获得。噬菌体X-2在形态上与先前描述的宿主范围非常广泛的丝状噬菌体X不同,噬菌体X也能裂解携带质粒R6K的菌株,并且这两种噬菌体对二乙醚灭活的抗性也不同。噬菌体X-2与噬菌体X以及类似X的噬菌体IKe和I2-2在血清学上没有关系。无法确定该噬菌体在携带质粒的菌株上的吸附位点,但提供了涉及接合菌毛的证据。