Bradley D E, Whelan J
Faculty of Medicine, Memorial University of Newfoundland, St John's, Canada.
J Gen Microbiol. 1989 Jul;135(7):1857-63. doi: 10.1099/00221287-135-7-1857.
The tolQ (previously fii) mutation in Escherichia coli K12 inhibits infection by filamentous bacteriophages f1 and IKe but not by RNA-containing phage f2. This work extends these observations to other plasmid-specific bacteriophages including various filamentous. RNA-containing, and lipid-containing isolates. Only tip-adsorbing filamentous phages were affected by tolQ and not shaft-adsorbing ones. Electron microscopy showed that RP4-specific filamentous phage Pf3 was one of the latter kind. Several tip-adsorbing filamentous phages inhibited conjugation between tolQ strains carrying their specific plasmids, implicating the phage receptors (conjugative pili) as mating organelles. tolQ mutant strains were as proficient as their parents in conjugation mediated by a wide range of plasmids.
大肠杆菌K12中的tolQ（以前称为fii）突变抑制丝状噬菌体f1和IKe的感染，但不抑制含RNA噬菌体f2的感染。这项工作将这些观察结果扩展到其他质粒特异性噬菌体，包括各种丝状、含RNA和含脂质的分离株。只有顶端吸附的丝状噬菌体受tolQ影响，而轴吸附的噬菌体不受影响。电子显微镜显示，RP4特异性丝状噬菌体Pf3属于后者。几种顶端吸附的丝状噬菌体抑制携带其特异性质粒的tolQ菌株之间的接合，这表明噬菌体受体（接合菌毛）作为交配细胞器。tolQ突变菌株在由多种质粒介导的接合中与其亲本一样熟练。
