García Elisa, Rodríguez-Barrera Roxana, Buzoianu-Anguiano Vinnitsa, Flores-Romero Adrian, Malagón-Axotla Emanuel, Guerrero-Godinez Marco, De la Cruz-Castillo Estefanía, Castillo-Carvajal Laura, Rivas-Gonzalez Monserrat, Santiago-Tovar Paola, Morales Ivis, Borlongan Cesar, Ibarra Antonio
Centro de Investigación en Ciencias de la Salud (CICSA), Facultad de Ciencias de la Salud, Universidad Anáhuac México Campus Norte, Huixquilucan, Edo. de México, México; Centro de Investigación del Proyecto CAMINA A.C.; Ciudad de México, México.
Unidad de Investigación Médica en Enfermedades Neurologicas, Hospital Especialidades CMN Siglo XXI, Ciudad de México, Mexico.
Neural Regen Res. 2019 Jun;14(6):1060-1068. doi: 10.4103/1673-5374.250627.
Spinal cord injury is a very common pathological event that has devastating functional consequences in patients. In recent years, several research groups are trying to find an effective therapy that could be applied in clinical practice. In this study, we analyzed the combination of different strategies as a potential therapy for spinal cord injury. Immunization with neural derived peptides (INDP), inhibition of glial scar formation (dipyridyl: DPY), as well as the use of biocompatible matrix (fibrin glue: FG) impregnated with bone marrow mesenchymal stem cells (MSCs) were combined and then its beneficial effects were evaluated in the induction of neuroprotection and neuroregeneration after acute SCI. Sprague-Dawley female rats were subjected to a moderate spinal cord injury and then randomly allocated into five groups: 1) phosphate buffered saline; 2) DPY; 3) INDP + DPY; 4) DPY+ FG; 5) INDP + DPY + FG + MSCs. In all rats, intervention was performed 72 hours after spinal cord injury. Locomotor and sensibility recovery was assessed in all rats. At 60 days after treatment, histological examinations of the spinal cord (hematoxylin-eosin and Bielschowsky staining) were performed. Our results showed that the combination therapy (DPY+ INDP + FG + MSCs) was the best strategy to promote motor and sensibility recovery. In addition, significant increases in tissue preservation and axonal density were observed in the combination therapy group. Findings from this study suggest that the combination theapy (DPY+ INDP + FG + MSCs) exhibits potential effects on the protection and regeneration of neural tissue after acute spinal cord injury. All procedures were approved by the Animal Bioethics and Welfare Committee (approval No. 178544; CSNBTBIBAJ 090812960) on August 15, 2016.
脊髓损伤是一种非常常见的病理事件，会给患者带来严重的功能后果。近年来，多个研究团队试图找到一种可应用于临床实践的有效治疗方法。在本研究中，我们分析了不同策略的组合作为脊髓损伤潜在治疗方法的效果。将神经源性肽免疫接种（INDP）、抑制胶质瘢痕形成（联吡啶：DPY）以及使用浸渍有骨髓间充质干细胞（MSCs）的生物相容性基质（纤维蛋白胶：FG）相结合，然后评估其在急性脊髓损伤后诱导神经保护和神经再生方面的有益效果。将雌性Sprague-Dawley大鼠造成中度脊髓损伤，然后随机分为五组：1）磷酸盐缓冲盐水；2）DPY；3）INDP + DPY；4）DPY + FG；5）INDP + DPY + FG + MSCs。在所有大鼠中，脊髓损伤72小时后进行干预。评估所有大鼠的运动和感觉恢复情况。治疗60天后，对脊髓进行组织学检查（苏木精-伊红染色和 Bielschowsky 染色）。我们的结果表明，联合治疗（DPY + INDP + FG + MSCs）是促进运动和感觉恢复的最佳策略。此外，联合治疗组的组织保存和轴突密度显著增加。本研究结果表明，联合治疗（DPY + INDP + FG + MSCs）对急性脊髓损伤后神经组织的保护和再生具有潜在作用。所有程序均于2016年8月15日获得动物生物伦理与福利委员会批准（批准号178544；CSNBTBIBAJ 090812960）。
