常见的 IL-10 启动子基因变异与突尼斯头颈部癌症易感性的关联。
Association of common IL-10 promoter gene variants with the susceptibility to head and neck cancer in Tunisia.
出版信息
Turk J Med Sci. 2019 Feb 11;49(1):123-128. doi: 10.3906/sag-1805-21.
BACKGROUND/AIM: We investigated the association of three IL-10 promoter single-nucleotide polymorphisms and altered IL-10 plasma levels with the risk of head and neck cancer (HNC).
MATERIALS AND METHODS
Study subjects comprised 194 HNC patients [137 nasopharyngeal cancer (NPC) and 57 laryngeal cancer (LC)], and 263 healthy controls. Genotyping of rs1800896 (-1082A>G), rs1800871 (-819C>T), and rs1800872 (-592A>C) IL-10 variants was performed by real-time PCR; IL-10 levels were measured by enzyme amplified immuno sensitivity assay (EAISA).
RESULTS
Study subjects comprised 194 HNC patients [137 nasopharyngeal cancer (NPC) and 57 laryngeal cancer (LC)], and 263 healthy controls. Genotyping of rs1800896 (-1082A>G), rs1800871 (-819C>T), and rs1800872 (-592A>C) IL-10 variants was performed by real-time PCR; IL-10 levels were measured by enzyme amplified immuno sensitivity assay (EAISA).
CONCLUSION
Our results demonstrate that IL-10-1082, IL-10-819, and IL-10-592 variants, and haplotypes GC and GT constitute biomarkers for early detection of HNC, especially NPC subtype. IL-10 -819T/C and TA haplotype may be used as biomarkers for early detection of LC.
背景/目的：我们研究了三个白细胞介素 10（IL-10）启动子单核苷酸多态性与白细胞介素 10 血浆水平改变与头颈部癌症（HNC）风险之间的关联。
材料和方法
研究对象包括 194 例 HNC 患者[137 例鼻咽癌（NPC）和 57 例喉癌（LC）]和 263 例健康对照。采用实时 PCR 法对 IL-10 变异 rs1800896（-1082A>G）、rs1800871（-819C>T）和 rs1800872（-592A>C）进行基因分型；采用酶放大免疫敏感性测定法（EAISA）检测 IL-10 水平。
结果
研究对象包括 194 例 HNC 患者[137 例鼻咽癌（NPC）和 57 例喉癌（LC）]和 263 例健康对照。采用实时 PCR 法对 IL-10 变异 rs1800896（-1082A>G）、rs1800871（-819C>T）和 rs1800872（-592A>C）进行基因分型；采用酶放大免疫敏感性测定法（EAISA）检测 IL-10 水平。
结论
我们的研究结果表明，IL-10-1082、IL-10-819 和 IL-10-592 变异体以及 GC 和 GT 单倍型可作为 HNC，尤其是 NPC 亚型的早期检测标志物。IL-10-819T/C 和 TA 单倍型可作为 LC 早期检测的标志物。
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