Department of Otorhinolaryngology-Head and Neck Surgery, School of Medicine, University of Occupational and Environmental Health, Kitakyushu, Japan.
Department of Otorhinolaryngology, Hanoi Medical University, Hanoi, Vietnam.
Clin Otolaryngol. 2019 May;44(3):356-365. doi: 10.1111/coa.13312. Epub 2019 Mar 18.
We investigated the difference in ciliary beat responsiveness to acetylcholine in ex vivo and the difference in the expressions of associated molecules (M1/M3 muscarinic receptors, pannexin-1 and P2X7 purinergic receptor) between the nasal polyp and turbinate mucosa.
Laboratorial study.
Nasal polyp and inferior turbinate were collected from patients with hypertrophic rhinitis and/or nasal polyp during endoscopic sinonasal surgery.
The mucosa was cut into thin strips, and ciliary movement was observed under a phase-contrast light microscope equipped with a high-speed digital video camera. The samples were also examined by scanning electron microscopy, fluorescence immunohistochemistry, and quantitative reverse transcription-polymerase chain reaction.
Cilia were well preserved in both tissues at the ultrastructural level. The baseline ciliary beat frequency (CBF) was not different between the two tissues. The CBF of the turbinate was significantly increased by stimulation with acetylcholine (P < 0.001), but that of the polyp was not. The ratio of the acetylcholine-stimulated CBF to the baseline CBF was significantly lower in the polyp than in the turbinate (P < 0.001). Immunohistochemical study revealed that immunoreactivities for M3, pannexin-1 and P2X7 were weaker in the polyp than in the turbinate. The mRNA expressions of M1, M3 and P2X7 were significantly lower and that of pannexin-1 tended to be lower in the polyp than in the turbinate.
These results indicate that ciliary beat responsiveness to acetylcholine is decreased in the nasal polyp. This may be explained by the decreased expressions of M3, P2X7 and probably pannexin-1 in this tissue.
研究鼻息肉和鼻甲黏膜之间乙酰胆碱刺激下纤毛摆动反应的差异，以及相关分子（M1/M3 毒蕈碱受体、连接蛋白-1 和 P2X7 嘌呤能受体）表达的差异。
实验室研究。
鼻甲和鼻息肉均取自接受内镜鼻窦手术的肥厚性鼻炎和/或鼻息肉患者。
将黏膜切成薄片，在配备高速数字摄像机的相差光显微镜下观察纤毛运动。还通过扫描电子显微镜、荧光免疫组织化学和定量逆转录聚合酶链反应对标本进行检查。
超微结构水平下，两种组织的纤毛均保存良好。基线纤毛摆动频率（CBF）在两种组织之间无差异。乙酰胆碱刺激下鼻甲的 CBF 显著增加（P<0.001），而鼻息肉则不然。与基线 CBF 相比，鼻息肉中乙酰胆碱刺激的 CBF 比值明显较低（P<0.001）。免疫组织化学研究显示，鼻息肉中 M3、连接蛋白-1 和 P2X7 的免疫反应性较鼻甲弱。与鼻甲相比，鼻息肉中 M1、M3 和 P2X7 的 mRNA 表达明显降低，而连接蛋白-1 的 mRNA 表达则趋于降低。
这些结果表明鼻息肉中乙酰胆碱刺激下纤毛摆动反应降低。这可能是由于该组织中 M3、P2X7 和可能的连接蛋白-1 的表达减少所致。
