Nikitin N S, Kuznetsov S L
Federal Research Centre of Nutrition, Biotechnology and Food Safety, Moscow.
I.M. Sechenov First Moscow State Medical University.
Vopr Pitan. 2018;87(6):16-21. doi: 10.24411/0042-8833-2018-10062. Epub 2018 Nov 13.
The aim of this study was to examine the effect of polyphenol quercetin on morphological changes in nonalcoholic fatty liver disease (NAFLD) in rats fed high-fructose diet. For 20 weeks animals (n=8 in each group) of the 1st group were given standard diet and water; the 2nd group - standard diet and 20% fructose solution; the 3rd group - standard diet with quercetin supplementation (0.1%) and 20% fructose solution. Formalin-fixed and paraffin-embedded liver samples were sectioned, stained (hematoxilin and eosin, Van Gieson's stain), evaluated with the use of the SAF and NAS scales. Histological assessment did not reveal pathology in the structure of the liver of the 1st group rats (S0A0F0; NAS - 0, fibrosis - 0). The 2nd group rat livers disclosed micro-, mid- and macrovesicular steatosis, inflammation without ballooning, pericellular and periportal fibrosis (S2A1F2; NAS - 3, fibrosis - 2). Quercetintreated rats exhibited in liver significantly less steatosis without significant changes in inflammation and fibrosis features (S1A1F2; NAS - 2, fibrosis - 2) compared with rats of the 2nd group. The data obtained demonstrate the ability of quercetin to inhibit the development of NAFLD in rats fed a diet with a high content of fructose, by reducing the severity of hepatostatosis.
本研究的目的是检验多酚槲皮素对喂食高果糖饮食的大鼠非酒精性脂肪性肝病（NAFLD）形态学变化的影响。20周内，第1组动物（每组n = 8）给予标准饮食和水；第2组给予标准饮食和20%果糖溶液；第3组给予补充槲皮素（0.1%）的标准饮食和20%果糖溶液。将用福尔马林固定并石蜡包埋的肝脏样本切片，染色（苏木精和伊红染色、范吉森染色），使用SAF和NAS量表进行评估。组织学评估未发现第1组大鼠肝脏结构有病变（S0A0F0；NAS - 0，纤维化 - 0）。第2组大鼠肝脏出现微泡、中泡和大泡性脂肪变性，无气球样变的炎症，细胞周围和汇管区纤维化（S2A1F2；NAS - 3，纤维化 - 2）。与第2组大鼠相比，槲皮素处理的大鼠肝脏脂肪变性明显减轻，炎症和纤维化特征无显著变化（S1A1F2；NAS - 2，纤维化 - 2）。获得的数据表明，槲皮素能够通过降低肝脂肪变性的严重程度，抑制喂食高果糖饮食大鼠NAFLD的发展。
