对 PANA RealTyper™ CRE 试剂盒检测革兰氏阴性杆菌碳青霉烯酶基因的性能评估。

Performance evaluation of the PANA RealTyper™ CRE Kit for detecting carbapenemase genes in Gram-negative bacilli.

机构信息

Department of Laboratory Medicine and Research Institute of Bacterial Resistance, College of Medicine, Yonsei University, Seoul, South Korea.

出版信息

J Glob Antimicrob Resist. 2019 Sep;18:100-103. doi: 10.1016/j.jgar.2019.02.002. Epub 2019 Feb 11.

DOI:10.1016/j.jgar.2019.02.002

PMID:30763759

Abstract

OBJECTIVES

The spread of carbapenemase-producing organisms has been continuously reported over the past decade. Rapid and accurate detection of carbapenemase production is essential for adequate infection control and appropriate antimicrobial treatment in the clinical setting. In this study, the performance of the newly developed PANA RealTyper™ CRE Kit (PANAGENE, Daejeon, South Korea) for the detection of six common carbapenemase genes (bla, bla, bla, bla, bla and bla) was evaluated.

METHODS

A total of 479 non-duplicate clinical isolates of Gram-negative bacilli, including 391 carbapenemase-producers and 88 non-producers, were tested. Conventional PCR and sequencing were performed as reference methods for performance evaluation of the PANA RealTyper™ CRE Kit.

RESULTS

The PANA RealTyper™ CRE Kit showed a reliable performance for the detection of carbapenemase-producing organisms compared with the monoplex PCR system, with sensitivity and specificity values both >95%, with only a few discrepant results for six types of carbapenemase genes.

CONCLUSION

This kit is rapid and accurate for simultaneously detecting various carbapenemase genes in a single reaction and could contribute to early decisions for appropriate antimicrobial treatment in the clinical setting.

摘要

目的

在过去的十年中，不断有关于碳青霉烯酶产生菌传播的报道。在临床环境中，快速准确地检测碳青霉烯酶的产生对于充分的感染控制和适当的抗菌治疗至关重要。在这项研究中，评估了新开发的 PANA RealTyper™ CRE 试剂盒（韩国大田的 PANAGENE）检测六种常见碳青霉烯酶基因（bla、bla、bla、bla、bla 和 bla）的性能。