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本文引用的文献

1
Detection of carbapenemases in Enterobacteriaceae by a commercial multiplex PCR.采用商业多重 PCR 检测肠杆菌科的碳青霉烯酶。
J Clin Microbiol. 2012 Sep;50(9):3115-8. doi: 10.1128/JCM.00991-12. Epub 2012 Jul 11.
2
Detection of extended-spectrum β-lactamase and Klebsiella pneumoniae Carbapenemase genes directly from blood cultures by use of a nucleic acid microarray.利用核酸微阵列直接从血培养物中检测超广谱β-内酰胺酶和肺炎克雷伯菌碳青霉烯酶基因。
J Clin Microbiol. 2012 Sep;50(9):2901-4. doi: 10.1128/JCM.01023-12. Epub 2012 Jun 20.
3
Evaluation of the New NucliSENS EasyQ KPC test for rapid detection of Klebsiella pneumoniae carbapenemase genes (blaKPC).评价新型 NucliSENS EasyQ KPC 检测试剂盒快速检测产碳青霉烯酶肺炎克雷伯菌（blaKPC）基因。
J Clin Microbiol. 2012 Aug;50(8):2783-5. doi: 10.1128/JCM.00284-12. Epub 2012 May 23.
4
Evaluation of a DNA microarray for the rapid detection of extended-spectrum β-lactamases (TEM, SHV and CTX-M), plasmid-mediated cephalosporinases (CMY-2-like, DHA, FOX, ACC-1, ACT/MIR and CMY-1-like/MOX) and carbapenemases (KPC, OXA-48, VIM, IMP and NDM).用于快速检测超广谱β-内酰胺酶（TEM、SHV 和 CTX-M）、质粒介导头孢菌素酶（CMY-2 样、DHA、FOX、ACC-1、ACT/MIR 和 CMY-1 样/MOX）和碳青霉烯酶（KPC、OXA-48、VIM、IMP 和 NDM）的 DNA 微阵列的评估。
J Antimicrob Chemother. 2012 Aug;67(8):1865-9. doi: 10.1093/jac/dks156. Epub 2012 May 17.
5
Identification and screening of carbapenemase-producing Enterobacteriaceae.碳青霉烯酶肠杆菌科的鉴定和筛选。
Clin Microbiol Infect. 2012 May;18(5):432-8. doi: 10.1111/j.1469-0691.2012.03815.x.
6
A loop-mediated isothermal amplification method for rapid detection of NDM-1 gene.一种用于快速检测 NDM-1 基因的环介导等温扩增方法。
Microb Drug Resist. 2012 Aug;18(4):359-63. doi: 10.1089/mdr.2011.0220. Epub 2012 Mar 14.
7
Detection of carbapenemase-producing Enterobacteriaceae with a commercial DNA microarray.采用商用 DNA 微阵列检测产碳青霉烯酶肠杆菌科细菌。
J Med Microbiol. 2012 Jun;61(Pt 6):809-812. doi: 10.1099/jmm.0.041673-0. Epub 2012 Mar 1.
8
Ultrarapid detection of blaKPC₁/₂-₁₂ from perirectal and nasal swabs by use of real-time PCR.实时 PCR 法快速检测直肠和鼻腔拭子中的 blaKPC₁/₂-₁₂。
J Clin Microbiol. 2012 May;50(5):1718-20. doi: 10.1128/JCM.00195-12. Epub 2012 Feb 29.
9
Rapid detection of carbapenemase genes by multiplex real-time PCR.多重实时 PCR 快速检测碳青霉烯酶基因。
J Antimicrob Chemother. 2012 Apr;67(4):906-9. doi: 10.1093/jac/dkr563. Epub 2012 Jan 9.
10
Value of the modified Hodge test for detection of emerging carbapenemases in Enterobacteriaceae.改良 Hodge 试验检测肠杆菌科中新型碳青霉烯酶的价值。
J Clin Microbiol. 2012 Feb;50(2):477-9. doi: 10.1128/JCM.05247-11. Epub 2011 Nov 23.

快速同时检测革兰氏阴性杆菌中产碳青霉烯酶肺炎克雷伯菌（blaKPC）和新德里金属β-内酰胺酶（blaNDM）的基因。

Rapid and simultaneous detection of genes encoding Klebsiella pneumoniae carbapenemase (blaKPC) and New Delhi metallo-β-lactamase (blaNDM) in Gram-negative bacilli.

机构信息

Division of Clinical Microbiology, Department of Laboratory Medicine and Pathology, Mayo Clinic, Rochester, Minnesota, USA.

出版信息

J Clin Microbiol. 2013 Apr;51(4):1269-71. doi: 10.1128/JCM.03062-12. Epub 2013 Jan 23.

DOI:10.1128/JCM.03062-12
PMID:23345290
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC3666784/
Abstract

We present a duplex, real-time PCR assay for detection of Klebsiella pneumoniae carbapenemase (blaKPC) and New Delhi metallo-β-lactamase (blaNDM) genes. Accuracy was assessed with 158 Gram-negative bacillary isolates, including 134 carbapenemase producers. The assay had 100% sensitivity and specificity compared with reference methods and a turnaround time of 90 min.

摘要

我们介绍了一种用于检测肺炎克雷伯菌碳青霉烯酶（blaKPC）和新德里金属β-内酰胺酶（blaNDM）基因的双管实时 PCR 检测方法。通过 158 株革兰氏阴性杆菌分离株（包括 134 株碳青霉烯酶产生菌）评估了该方法的准确性。与参考方法相比，该方法的检测灵敏度和特异性均为 100%，检测周转时间为 90 分钟。