快速同时检测革兰氏阴性杆菌中产碳青霉烯酶肺炎克雷伯菌（blaKPC）和新德里金属β-内酰胺酶（blaNDM）的基因。
Rapid and simultaneous detection of genes encoding Klebsiella pneumoniae carbapenemase (blaKPC) and New Delhi metallo-β-lactamase (blaNDM) in Gram-negative bacilli.
机构信息
Division of Clinical Microbiology, Department of Laboratory Medicine and Pathology, Mayo Clinic, Rochester, Minnesota, USA.
出版信息
J Clin Microbiol. 2013 Apr;51(4):1269-71. doi: 10.1128/JCM.03062-12. Epub 2013 Jan 23.
Abstract
We present a duplex, real-time PCR assay for detection of Klebsiella pneumoniae carbapenemase (blaKPC) and New Delhi metallo-β-lactamase (blaNDM) genes. Accuracy was assessed with 158 Gram-negative bacillary isolates, including 134 carbapenemase producers. The assay had 100% sensitivity and specificity compared with reference methods and a turnaround time of 90 min.
摘要
我们介绍了一种用于检测肺炎克雷伯菌碳青霉烯酶（blaKPC）和新德里金属β-内酰胺酶（blaNDM）基因的双管实时 PCR 检测方法。通过 158 株革兰氏阴性杆菌分离株（包括 134 株碳青霉烯酶产生菌）评估了该方法的准确性。与参考方法相比，该方法的检测灵敏度和特异性均为 100%，检测周转时间为 90 分钟。
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