Department of Microbiology, School of Medicine, Miyazaki University, Miyazaki, Japan.
Department of Electron Microscopy and Histopathology, Noguchi Memorial Institute for Medical Research, College of Health Sciences, University of Ghana, Legon, Accra, Ghana; Department of Molecular Epidemiology, Graduate School of Biomedical Sciences, Nagasaki University, Nagasaki, Japan.
Infect Genet Evol. 2019 Apr;69:246-254. doi: 10.1016/j.meegid.2019.02.007. Epub 2019 Feb 11.
Rotavirus A (RVA) causes acute diarrhoea in children and less frequently in adults. However, the knowledge about the genotype distribution of RVA strains circulating in adults is limited particularly in developing countries. This study aimed to characterise the RVA strains detected from adult patients with diarrhoea in Nepal. A total of 47 RVA positive stool samples from adult patients with diarrhoea in Kathmandu, Nepal during 2007-2008 were examined for the G and P genotypes by sequencing. Nearly half (49%) of the samples were genotyped as G9P[8] (n = 23), G1P[8], G2P[4] (n = 5 each), G12P[8] (n = 4), G12P[6] (n = 3), G1P[6] (n = 2), G3P[8] and G9P[6] (n = 1 each). Interestingly, two G11P[25] and one G9P[19] strains detected were further subjected to Illumina MiSeq next generation sequencing to determine their whole genome sequences. The genotype constellations of RVA/Human-wt/NPL/TK2615/2008/G11P[25] and RVA/Human-wt/NPL/TK2620/2008/G11P[25] were I12-R1-C1-M1-A1-N1-T1-E1-H1, whereas that of RVA/Human-wt/NPL/TK1797/2007/G9P[19] was I5-R1-C1-M1-A8-N1-T1-E1-H1. The 11 genes of TK2615 and TK2620 were virtually identical, and they were either porcine-like or unique except the VP2 and NSP1 genes which were of human RVA origin. The two G11P[25] strains were also very similar to KTM368, another G11P[25] isolated from a child in Nepal in 2004. On the other hand, no gene of TK1797 was likely to be of human RVA origin. The observation that porcine-like RVAs were detected from adult patients justifies further studies to explore the role of adults in the interspecies transmission of animal RVA to humans.
轮状病毒 A（RVA）可导致儿童急性腹泻，在成人中也较少发生。然而，有关在发展中国家循环的 RVA 株的基因型分布的知识有限。本研究旨在描述尼泊尔成人腹泻患者中检测到的 RVA 株的特征。2007-2008 年，对来自尼泊尔加德满都的 47 例成人腹泻患者的 47 份 RVA 阳性粪便样本进行了 G 和 P 基因型测序。将近一半（49%）的样本被鉴定为 G9P[8]（n=23）、G1P[8]、G2P[4]（n=5 例）、G12P[8]（n=4）、G12P[6]（n=3）、G1P[6]（n=2）、G3P[8]和 G9P[6]（n=1）。有趣的是，检测到的两株 G11P[25]和一株 G9P[19]进一步通过 Illumina MiSeq 下一代测序来确定它们的全基因组序列。RVA/Human-wt/NPL/TK2615/2008/G11P[25]和 RVA/Human-wt/NPL/TK2620/2008/G11P[25]的基因型组合为 I12-R1-C1-M1-A1-N1-T1-E1-H1，而 RVA/Human-wt/NPL/TK1797/2007/G9P[19]的基因型组合为 I5-R1-C1-M1-A8-N1-T1-E1-H1。TK2615 和 TK2620 的 11 个基因几乎相同，除了 VP2 和 NSP1 基因源自人类 RVA 外，它们要么是猪源样的，要么是独特的。两株 G11P[25]与 2004 年从尼泊尔儿童中分离的另一株 G11P[25]的 KTM368 也非常相似。另一方面，TK1797 的基因不太可能源自人类 RVA。从成人患者中检测到猪源样 RVAs 的观察结果表明，有必要进一步研究探索成人在动物 RVA 向人类的种间传播中的作用。
