Navarro Ryan, Aung Meiji Soe, Cruz Katalina, Ketzis Jennifer, Gallagher Christa Ann, Beierschmitt Amy, Malik Yashpal Singh, Kobayashi Nobumichi, Ghosh Souvik
Department of Biomedical Sciences, Ross University School of Veterinary Medicine, Saint Kitts and Nevis, West Indies.
Department of Hygiene, Sapporo Medical University, Sapporo, Hokkaido, Japan.
Infect Genet Evol. 2017 Apr;49:21-31. doi: 10.1016/j.meegid.2016.12.026. Epub 2016 Dec 28.
We report here whole genome analysis of a porcine rotavirus-A (RVA) strain RVA/Pig-wt/KNA/ET8B/2015/G5P[13] detected in a diarrheic piglet, and nearly whole genome (except for VP4 gene) analysis of a simian RVA strain RVA/Simian-wt/KNA/08979/2015/G5P[X] detected in a non-diarrheic African green monkey (AGM) on the island of St. Kitts, Caribbean region. Strain ET8B exhibited a G5-P[13]-I5-R1-C1-M1-A8-N1-T7-E1-H1 genotype constellation that was identical to those of Brazilian porcine RVA G5P[13] strains RVA/Pig-wt/BRA/ROTA01/2013/G5P[13] and RVA/Pig-wt/BRA/ROTA07/2013/G5P[13], the only porcine G5P[13] RVAs that have been analyzed for the whole genome so far. Phylogenetically, all the 11 gene segments of ET8B were closely related to those of porcine and porcine-like human RVAs within the respective genotypes. Although the porcine G5P[13] RVAs exhibited identical genotype constellations, ET8B did not appear to share common evolutionary pathways with the Brazilian porcine G5P[13] RVAs. Interestingly, the VP2, VP3, VP6, VP7, and NSP1-NSP5 genes of simian RVA strain 08979 were closely related to those of porcine and porcine-like human RVA strains, exhibiting 99%-100% nucleotide sequence identities to cognate genes of co-circulating porcine RVA strain ET8B. On the other hand, the VP1 of 08979 appeared to be genetically divergent from porcine and human RVAs within the R1 genotype, and its exact origin could not be ascertained. Taken together, these observations suggested that simian strain 08979 might have been derived from interspecies transmission events involving transmission of ET8B-like RVAs from pigs to AGMs. In St. Kitts, AGMs often stray from the wild into livestock farms. Therefore, it may be possible that the AGM acquired the infection from a pig farm on the island. To our knowledge, this is the first report on detection of porcine-like RVAs in monkeys. Also, the present study is the first to report whole genomic analysis of a porcine RVA strain from the Caribbean region.
我们在此报告对一株从腹泻仔猪中检测到的猪轮状病毒A（RVA）毒株RVA/Pig-wt/KNA/ET8B/2015/G5P[13]进行的全基因组分析，以及对一株从加勒比地区圣基茨岛一只未腹泻的非洲绿猴（AGM）中检测到的猿RVA毒株RVA/Simian-wt/KNA/08979/2015/G5P[X]进行的近乎全基因组（除VP4基因外）分析。ET8B毒株呈现出G5-P[13]-I5-R1-C1-M1-A8-N1-T7-E1-H1基因型组合，与巴西猪RVA G5P[13]毒株RVA/Pig-wt/BRA/ROTA01/2013/G5P[13]和RVA/Pig-wt/BRA/ROTA07/2013/G5P[13]相同，这是迄今为止仅有的两株已进行全基因组分析的猪G5P[13] RVAs。在系统发育上，ET8B的所有11个基因片段在各自基因型内与猪和猪样人RVA的基因片段密切相关。尽管猪G�P[13] RVAs呈现出相同的基因型组合，但ET8B似乎与巴西猪G5P[13] RVAs没有共同的进化途径。有趣的是，猿RVA毒株08979的VP2、VP3、VP6、VP7和NSP1-NSP5基因与猪和猪样人RVA毒株的这些基因密切相关，与共同流行的猪RVA毒株ET8B的同源基因核苷酸序列同一性达99%-100%。另一方面，08979的VP1在R1基因型内似乎在基因上与猪和人RVA不同，其确切来源无法确定。综合这些观察结果表明，猿毒株08979可能源自种间传播事件，涉及类似ET8B的RVAs从猪传播到AGMs。在圣基茨，AGMs经常从野外进入畜牧场。因此，AGM有可能是从该岛的一个养猪场感染的。据我们所知，这是关于在猴子中检测到猪样RVAs的首次报告。此外，本研究也是首次报告来自加勒比地区的一株猪RVA毒株的全基因组分析。
