Research Institute of Forestry; State Key Laboratory of Tree Genetics and Breeding, Chinese Academy of Forestry, Beijing 100091, China.
Int J Mol Sci. 2019 Feb 5;20(3):688. doi: 10.3390/ijms20030688.
, an epiphytic and lithophytic species, suffers frequently from perennial shortage of water in the wild. The molecular mechanisms of this orchid's tolerance to abiotic stress, especially drought, remain largely unknown. It is well-known that CBL-interacting protein kinase (CIPKs) proteins play important roles in plant developmental processes, signal transduction, and responses to abiotic stress. To study the CIPKs' functions for , we first identified 24 CIPK genes from it. We divided them into three subgroups, with varying intron numbers and protein motifs, based on phylogeny analysis. Expression patterns of CIPK family genes in different tissues and in response to either drought or cold stresses suggested may be associated with signal transduction and energy metabolism. , -, and - are predicted to play critical roles during drought treatment specifically. Furthermore, transcript expression abundances of showed polar opposites during day and night. Whether under drought treatment or not, tended to emphatically express transcript1 during the day and transcript3 at night. This implied that expression of the transcripts might be regulated by circadian rhythm. qRT-PCR analysis also indicated that , -, and - were strongly influenced by circadian rhythmicity. In contrast with previous studies, for the first time to our knowledge, our study revealed that the major CIPK gene transcript expressed was not always the same and was affected by the biological clock, providing a different perspective on alternative splicing preference.
作为一种附生和石生的物种，它在野外经常遭受常年缺水的困扰。这种兰花对非生物胁迫，特别是干旱的耐受机制在很大程度上仍然未知。众所周知，CBL 相互作用蛋白激酶（CIPKs）蛋白在植物发育过程、信号转导和对非生物胁迫的反应中发挥着重要作用。为了研究 CIPKs 对 的功能，我们首先从它中鉴定出 24 个 CIPK 基因。我们根据系统发育分析将它们分为三组，每组的内含子数量和蛋白基序不同。CIPK 家族基因在不同组织中的表达模式以及对干旱或冷胁迫的反应表明，它可能与信号转导和能量代谢有关。 、 、 和 被预测在干旱处理中发挥关键作用。此外，在昼夜表达模式中， 显示出极性相反的表达模式。无论是否受到干旱处理的影响， 白天倾向于强烈表达转录本 1，而夜间则强烈表达转录本 3。这表明转录本的表达可能受到昼夜节律的调节。qRT-PCR 分析还表明， 、 、 和 强烈受到昼夜节律的影响。与以前的研究不同，我们的研究首次揭示了主要的 CIPK 基因转录本并不总是相同的，并且受到生物钟的影响，为选择性剪接偏好提供了不同的视角。
