Department of Theriogenology, Faculty of Veterinary Science, University of Veterinary and Animal Sciences, Lahore 54000, Pakistan.
Nuclear Institute for Agriculture and Biology (NIAB), Faisalabad 38000, Pakistan.
Anim Reprod Sci. 2019 Mar;202:80-86. doi: 10.1016/j.anireprosci.2019.02.001. Epub 2019 Feb 8.
The objective of present study was to determine the effect of plasma progesterone (P4) on oocyte recovery, oocyte quality, and early in-vitro developmental competence of embryos in Bos indicus dairy cows. The ovaries were collected in an abattoir. These ovaries (n = 750) were divided into two groups: 1) estrous CYCLIC (n = 318), and 2) estrous ACYCLIC (n = 432). Mean serum concentrations of P4 in a subset of (n = 85; 4.21 ± 0.4 ng/ml compared with 0.5 ± 0.2 ng/ml; P < 0.05) were greater in estrous CYCLIC as compared to ACYCLIC cows, respectively. The mean number of oocytes recovered per ovary (6.5 ± 0.5 compared with 4.0 ± 0.2; P < 0.05) was greater for estrous CYCLIC than ACYCLIC cows, respectively. The oocytes with grade I_+II quality (55.3% compared with 47.6%; P < 0.05) were greater, whereas, there was lesser percentage with grade III+_IV quality (44.5% compared with 52.4%; P < 0.05) from estrous CYCLIC as compared with ACYCLIC cows, respectively. Cleavage rate (70.9% compared with 52.8%; P < 0.05) was greater for embryos derived from estrous CYCLIC than ACYCLIC cows, respectively. Similarly, the embryo developmental rates to the 8- (38.5% compared with 20.8%; P < 0.05) and 16- (20.0% compared with 10.9%; P < 0.05) cell stage were greater for embryos derived from estrous CYCLIC as compared to ACYCLIC cows, respectively. In conclusion, the presence of greater plasma P4 has a beneficial effect on oocyte recovery, oocyte quality, and early IVEP outcomes in Bos indicus dairy cows.
本研究旨在确定血浆孕酮（P4）对印度奶水牛卵母细胞回收、卵母细胞质量和早期体外胚胎发育能力的影响。这些卵巢（n=750）被分为两组：1）发情周期（n=318），2）发情非周期（n=432）。发情周期组（n=85）的血清 P4 浓度中位数（4.21±0.4ng/ml 比 0.5±0.2ng/ml；P<0.05）高于发情非周期组。发情周期组卵巢回收的卵母细胞数中位数（6.5±0.5 个比 4.0±0.2 个；P<0.05）多于发情非周期组。卵母细胞质量等级为 I_+II（55.3%比 47.6%；P<0.05）的比例更高，而质量等级为 III+_IV（44.5%比 52.4%；P<0.05）的比例更低。从发情周期组获得的胚胎的卵裂率（70.9%比 52.8%；P<0.05）更高。胚胎发育到 8-细胞（38.5%比 20.8%；P<0.05）和 16-细胞（20.0%比 10.9%；P<0.05）阶段的比率从发情周期组获得的胚胎更高。总之，血浆 P4 水平升高对印度奶水牛卵母细胞回收、卵母细胞质量和早期体外胚胎发育结果有有益影响。
