Lorenzo-Torres Alfredo, Rangel-Santos Raymundo, Bautista-Pérez Yuri Viridiana, González-Maldonado Juan
Departamento de Zootecnia, Universidad Autónoma Chapingo, Texcoco 56230, Mexico.
Instituto de Ciencias Agrícolas, Universidad Autónoma de Baja California, Mexicali 21705, Mexico.
Vet Sci. 2025 Jul 24;12(8):690. doi: 10.3390/vetsci12080690.
The corpus luteum (CL) is a transient gland that can directly influence follicular dynamics and oocyte quality. The objective of this study was to evaluate the influence of the absence or presence of a small (≤3 mm), medium (4-8 mm), or large (>8 mm) CL in slaughterhouse ovaries on in vitro embryo production. Cumulus-oocyte complexes (COCs) were collected from each group of ovaries and matured in TCM-199 medium, plus hormones and fetal bovine serum. Fertilization was performed with fresh semen from a Katahdin ram of known fertility. Embryo development was carried out in commercial sequential media for 72 and 96 h, until the blastocyst stage. The number of follicles (2-6 mm in diameter) and COCs were influenced by the presence of CL, which was higher ( < 0.05) in the Large CL group (5.51 ± 0.33 and 3.62 ± 0.27) compared to the Without CL group (4.54 ± 0.19 and 2.62 ± 0.14, respectively), with no difference between the CL sizes. Likewise, the diameter and area of the COCs were higher in the Small CL group of ovaries compared to the Without CL group. In the Large CL group of ovaries, 9% more morulae ( < 0.05) were obtained compared to the Without CL group; in the Medium CL group, 13% more blastocysts were obtained compared to the Without CL group. However, in the hatching capacity and diameter of blastocysts, no statistical difference was evident ( > 0.05). In conclusion, the presence and size of the CL in the ovaries of slaughtered sheep influence the productive efficiency of embryos in vitro under the conditions in which the present study was carried out.
黄体（CL）是一种临时性腺体，可直接影响卵泡动态和卵母细胞质量。本研究的目的是评估屠宰场卵巢中存在或不存在小（≤3毫米）、中（4 - 8毫米）或大（>8毫米）黄体对体外胚胎生产的影响。从每组卵巢中收集卵丘-卵母细胞复合体（COC），并在添加激素和胎牛血清的TCM - 199培养基中成熟。使用已知生育能力的卡他丁公羊的新鲜精液进行受精。胚胎发育在商业序贯培养基中进行72和96小时，直至囊胚阶段。卵泡（直径2 - 6毫米）和COC的数量受黄体存在的影响，与无黄体组（分别为4.54±0.19和2.62±0.14）相比，大黄体组（5.51±0.33和3.62±0.27）中的数量更高（<0.05），黄体大小之间无差异。同样，与无黄体组相比，小黄体组卵巢中的COC直径和面积更大。在大黄体组卵巢中，与无黄体组相比，桑葚胚数量多9%（<0.05）；在中黄体组中，与无黄体组相比，囊胚数量多13%。然而，在囊胚的孵化能力和直径方面，没有明显的统计学差异（>0.05）。总之，在本研究开展的条件下，屠宰羊卵巢中黄体的存在和大小会影响体外胚胎的生产效率。
