Laboratoire de recherche en inflammation et physiologie des granulocytes, Université du Québec, INRS-Institut Armand-Frappier, Laval, Québec, Canada.
Laboratoire de recherche en inflammation et physiologie des granulocytes, Université du Québec, INRS-Institut Armand-Frappier, Laval, Québec, Canada.
Cell Immunol. 2019 Mar;337:62-70. doi: 10.1016/j.cellimm.2019.02.002. Epub 2019 Feb 8.
The biological roles of interleukin (IL)-21 in human monocytes and macrophages have been neglected. We previously demonstrated that IL-21 induce phagocytosis and established that Syk is a new molecular target of IL-21. Herein, we found that IL-21 is not chemoattractant for immature THP-1 and primary monocytes but can increase the capacity of THP-1 cells (not primary monocytes) to adhere onto a cell substratum by a Syk-dependent mechanism without altering the expression of a panel of cell surface molecules. Unlike THP- 1 and monocytes, IL-21 can increase metalloproteinase (MMP)-9 secretion and activity in monocyte-derived macrophages (HMDM), as assessed by western blot and zymography experiments, respectively. We reported that IL-21 did not increase the production of IL-6 and the chemokines MIP-1α and GRO-α in HMDM. Therefore, IL-21 can increase functions other that phagocytosis, but this cytokine does not have a large spectrum of biological activities in monocytes and macrophages.
白细胞介素 (IL)-21 在人类单核细胞和巨噬细胞中的生物学作用一直被忽视。我们之前证明了 IL-21 诱导吞噬作用，并确定 Syk 是 IL-21 的一个新的分子靶标。在此，我们发现 IL-21 不是不成熟 THP-1 和原代单核细胞的趋化因子，但可以通过依赖于 Syk 的机制增加 THP-1 细胞（而不是原代单核细胞）黏附在细胞基质上的能力，而不改变一系列细胞表面分子的表达。与 THP-1 和单核细胞不同，IL-21 可以增加单核细胞来源的巨噬细胞 (HMDM) 中金属蛋白酶 (MMP)-9 的分泌和活性，分别通过 Western blot 和酶谱实验进行评估。我们报道说，IL-21 不会增加 HMDM 中 IL-6 和趋化因子 MIP-1α 和 GRO-α 的产生。因此，IL-21 可以增加吞噬作用以外的功能，但这种细胞因子在单核细胞和巨噬细胞中没有广泛的生物学活性。
