B-Box 蛋白 BBX30 和 BBX31 作为 HY5 的下游因子，负调控. 的光形态建成。
B-Box Containing Proteins BBX30 and BBX31, Acting Downstream of HY5, Negatively Regulate Photomorphogenesis in .
机构信息
Institute of Plant and Food Sciences, Department of Biology, Southern University of Science and Technology, Shenzhen 518055, China.
State Key Laboratory of Crop Genetics and Germplasm Enhancement, College of Agriculture, Nanjing Agricultural University, Nanjing 210095, China.
出版信息
Plant Physiol. 2019 May;180(1):497-508. doi: 10.1104/pp.18.01244. Epub 2019 Feb 14.
Abstract
Light-mediated seedling development is coordinately controlled by a variety of key regulators. Here, we identified two B-box (BBX)-containing proteins, BBX30 and BBX31, as repressors of photomorphogenesis. ELONGATED HYPOCOTYL5, a central regulator of light signaling, directly binds to the element present in the promoters of and and negatively controls their transcription levels in the light. Seedlings with mutations in or are hypersensitive to light, whereas the overexpression of or leads to hypo-photomorphogenic growth in the light. Furthermore, transgenic and phenotypic analysis revealed that the B-box domain of BBX30 or BBX31 is essential for their respective functioning in the regulation of photomorphogenic development in plants. In conclusion, BBX30 and BBX31 act as key negative regulators of light signaling, and their transcription is repressed by ELONGATED HYPOCOTYL5 through directly associating with their promoters.
摘要
光介导的幼苗发育是由多种关键调节剂协调控制的。在这里，我们鉴定了两个含有 B -box（BBX）的蛋白质，BBX30 和 BBX31，它们是光形态建成的抑制剂。ELONGATED HYPOCOTYL5 是光信号的中央调节剂，它直接结合到 和 启动子中存在的元件上，并在光下负调控它们的转录水平。在 或 中发生突变的幼苗对光敏感，而 或 的过表达导致光下的光形态发生生长缺陷。此外，转基因和表型分析表明，BBX30 或 BBX31 的 B-box 结构域对于它们在植物光形态建成发育调控中的各自功能是必需的。总之，BBX30 和 BBX31 作为光信号的关键负调节剂，其转录受到 ELONGATED HYPOCOTYL5 的抑制，通过直接与它们的启动子结合。
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