Department of Chemistry and Chemical Biology, University of New Mexico, Albuquerque, NM, 87131, USA.
UNM Comprehensive Cancer Center, University of New Mexico, Albuquerque, NM, 87131, USA.
Sci Rep. 2019 Feb 14;9(1):2102. doi: 10.1038/s41598-019-38511-z.
Detection of cellular senescence is important not only in the study of senescence in various biological systems, but also in various practical applications such as image-guided surgical removal of senescent cells, as well as the monitoring of drug-responsiveness during cancer therapies. Due to the lack of suitable imaging probes for senescence detection, particularly in living subjects, we have developed an activatable near-infrared (NIR) molecular probe with far-red excitation, NIR emission, and high "turn-on" ratio upon senescence-associated β-galactosidase (SABG) activation. We present here the first successful demonstration of NIR imaging of DNA damage-induced senescence both in vitro and in human tumor xenograft models.
细胞衰老的检测不仅在各种生物系统衰老的研究中很重要，而且在各种实际应用中也很重要，如通过图像引导进行衰老细胞的手术切除，以及监测癌症治疗过程中的药物反应性。由于缺乏用于衰老检测的合适成像探针，特别是在活体中，我们开发了一种具有远红色激发、近红外发射和高“开启”比的可激活近红外（NIR）分子探针，用于衰老相关β-半乳糖苷酶（SABG）的激活。我们在此首次成功地展示了 NIR 成像在体外和人肿瘤异种移植模型中诱导的 DNA 损伤诱导的衰老。
