Jourkesh Morteza, Soori Rahman, Earnest Conrad P, Mirheidari Lamia, Ravasi Ali Asghar, Stannard Stephen R, Monsalves-Alvarez Matias
Department of Physical Education and Sports Science, Shabestar Branch, Islamic Azad University, Iran.
Department of Exercise Physiology, Faculty of Physical Education and Sport Sciences, University of Tehran, Tehran, Iran.
Prz Menopauzalny. 2018 Dec;17(4):155-160. doi: 10.5114/pm.2018.81737. Epub 2018 Dec 30.
Heart disease risk rises with age. However, women's symptoms become more pronounced following the onset of menopause. The aim of the present study was to evaluate the effects of six weeks of combined resistance-endurance (RE) training on microRNA-29 expression in the heart of ovariectomised rats.
Thirty female Wistar rats were divided into three groups: 1) sham (SHAM); 2) ovariectomy (OVX); and 3) OVX with RE training (OVX + RE). The effects of these treatments on cardiac microRNA-29 expression were measured using real-time PCR. Data were analysed using a 2 × 3 ANOVA and Tukey comparisons and presented as mean ±SEM.
Ovariectomy resulted in a significant down-regulation in the heart microRNA-29 gene expression of OVX (0.265 ±0.031 fold changes), OVX + RE (0.699 ±0.038 fold changes) in animals vs. sham animals (1 ±0 fold changes; all, < 0.05) following six weeks of treatment. However, microRNA-29 expression in the OVX + RE group was significantly greater than in the OVX group ( < 0.05).
Our findings suggest that the six weeks of regular RE training attenuate the reduction in heart muscle microRNA-29 expression observed in ovariectomised rates. If our findings carry over to humans, such an exercise regimen could be beneficial to the cardiovascular disease risk in women during menopause.
心脏病风险随年龄增长而升高。然而，女性在绝经后症状会变得更加明显。本研究的目的是评估为期六周的抗阻耐力联合训练（RE）对去卵巢大鼠心脏中微小RNA-29表达的影响。
30只雌性Wistar大鼠分为三组：1）假手术组（SHAM）；2）去卵巢组（OVX）；3）去卵巢并接受RE训练组（OVX + RE）。使用实时PCR检测这些处理对心脏微小RNA-29表达的影响。数据采用2×3方差分析和Tukey比较进行分析，并以平均值±标准误表示。
六周治疗后，与假手术组动物（倍数变化为1±0；所有P<0.05）相比，去卵巢组（倍数变化为0.265±0.031）和去卵巢并接受RE训练组（倍数变化为0.699±0.038）的心脏微小RNA-29基因表达显著下调。然而，去卵巢并接受RE训练组的微小RNA-29表达显著高于去卵巢组（P<0.05）。
我们的研究结果表明，为期六周的规律RE训练可减轻去卵巢大鼠中心肌微小RNA-29表达的降低。如果我们的研究结果适用于人类，这样的运动方案可能对绝经后女性的心血管疾病风险有益。
