El-Masry Manal W, Gouda Heba M, Shaheen Iman A, Edesa Wael, Hassan Naglaa M, Ramzy Rehab
Department of Clinical and Chemical Pathology, Faculty of Medicine, Cairo University, Egypt.
Department of Clinical Oncology, National Cancer Institute, Cairo University, Cairo, Egypt.
Afr Health Sci. 2018 Dec;18(4):958-964. doi: 10.4314/ahs.v18i4.15.
Acute myeloid leukemia (AML), an aggressive clonal disease, is genetically heterozygous. The prognostic role of expression of Breast Cancer Resistance Protein (BCRP) gene, which behaves as a multidrug transporter, in adult AML is ambiguous.
The objective is to assess the level of mRNA expression of BCRP gene in newly diagnosed cytogenetically normal adult Egyptian AML patients; and to clarify its potential influence and association between therapeutic responsiveness and disease free survival.
The BCRP gene expression was evaluated by quantifying its mRNA using real time RT-PCR in fifty newly diagnosed cytogenetically normal adult AML patients and 20 healthy normal controls. The expression was evaluated in relation to clinical and prognostic factors, response to treatment and the survival rate.
BCRP mRNA was over expressed in adult AML patients compared to controls. This study showed a positive statistical correlation between BCRP gene expression and the percent of CD34 expression. Statistical analysis did not reveal any association between BCRP expression level and chemotherapeutic responsiveness or disease free survival rate.
The significance of BCRP gene expression and its function in AML is very complicated, therefore more standardized clinical studies are needed.
急性髓系白血病（AML）是一种侵袭性克隆性疾病，具有基因杂合性。乳腺癌耐药蛋白（BCRP）基因作为一种多药转运蛋白，其在成人AML中的预后作用尚不明确。
评估新诊断的细胞遗传学正常的成年埃及AML患者中BCRP基因的mRNA表达水平；阐明其对治疗反应性和无病生存的潜在影响及相关性。
采用实时逆转录聚合酶链反应（RT-PCR）定量检测50例新诊断的细胞遗传学正常的成年AML患者及20例健康正常对照者的BCRP基因mRNA表达，评估其与临床及预后因素、治疗反应和生存率的关系。
与对照组相比，成年AML患者中BCRP mRNA表达上调。本研究显示BCRP基因表达与CD34表达百分比之间存在正相关。统计学分析未显示BCRP表达水平与化疗反应性或无病生存率之间存在任何关联。
BCRP基因在AML中的表达及其功能的意义非常复杂，因此需要更多标准化的临床研究。
