Tabbabi Ahmed, Laamari Ali, Cheikh Raja Ben, Jha Ibtissem Ben, Daaboub Jabeur, Cheikh Hassen Ben
Laboratory of Genetics, Faculty of Medicine of Monastir, University of Monastir, Monastir, Tunisia.
Department of Hygiene and Environmental Protection, Ministry of Public Health, Tunis, Tunisia.
Afr Health Sci. 2018 Dec;18(4):1175-1181. doi: 10.4314/ahs.v18i4.38.
is an important vector of human diseases.
To determine the insecticide resistance development in against selection pressure of temephos..
A field population of was collected from Northwestern Tunisia with a medium level of temephos resistance (LC = 0.0069). It was subjected to six generations of temephos pressure selection to evaluate its relationship to cross-resistance towards organophosphates (OPs) and pyrethroids (PYR) insecticides.
The selection was initiated at the dose 0.0266, 0.0748 and 0.0069 which were increased during successive generations up to 0.1488, 3.8747 and 0.0086 after sixth generation for temephos, chlorpyrifos and permethrin insecticides, respectively. It is important to noted that high cross-resistance to chlorpyrifos insecticide (OP) was detected (51.88×). However, little or no cross-resistance to the pyrethroid permethrin (PYR) was recorded (1.24×). Contrary to metabolic resistance, it seemed that acetylcholinesterases AChE 1 was fixed under pressure selection.
The high cross-resistance to temephos and chlorpyrifos is reasonable because they belong to the same class of insecticide (OP). However, the little cross-resistance to the pyrethroid permethrin could support its use alternately for control.
是人类疾病的重要传播媒介。
确定在杀螟硫磷选择压力下的抗药性发展情况。
从突尼斯西北部收集了一个具有中等杀螟硫磷抗性水平（LC = 0.0069）的野外种群。对其进行了六代杀螟硫磷压力选择，以评估其与对有机磷（OPs）和拟除虫菊酯（PYR）类杀虫剂的交叉抗性之间的关系。
选择从剂量0.0266、0.0748和0.0069开始，在连续几代中增加，到第六代后，杀螟硫磷、毒死蜱和氯菊酯杀虫剂的剂量分别达到0.1488、3.8747和0.0086。需要注意的是，检测到对毒死蜱杀虫剂（OP）有高交叉抗性（51.88倍）。然而，对拟除虫菊酯氯菊酯（PYR）的交叉抗性很小或没有记录（1.24倍）。与代谢抗性相反，似乎乙酰胆碱酯酶AChE 1在压力选择下被固定。
对杀螟硫磷和毒死蜱的高交叉抗性是合理的，因为它们属于同一类杀虫剂（OP）。然而，对拟除虫菊酯氯菊酯的交叉抗性很小，这可能支持交替使用它来控制。
