Kim Hongrae, Lee Sunhye, Kim Hyunjin, Chang Hee Jin, Choi Yongdoo, Yoon Hong Man, Kook Myeong-Cherl, Jung So-Youn, Kwon Youngmi, Kang Juehyung, Yoo Hongki, Song Incheon, Lee Seungrag, Sohn Dae Kyung
Innovative Medical Engineering & Technology, National Cancer Center, Goyang, South Korea.
Biomarker Branch, National Cancer Center, Goyang, South Korea.
Microsc Res Tech. 2019 Jun;82(6):892-897. doi: 10.1002/jemt.23234. Epub 2019 Feb 14.
With the development of advanced and minimally invasive surgical techniques, and in view of the functional and cosmetic aspects, the need for rapid and accurate diagnosis during surgery is increasing. This study was conducted to develop a tissue diagnosis method using confocal microscopy after simple tissue staining that does not require freezing and slicing. At present, fluorescence staining with confocal microscopy is not generalized for real-time diagnosis during surgery. In this paper, we propose a fluorescence staining method using Hoechst 33342 and Eosin that does not require tissue freezing and slicing. The proposed method can be used as part of a rapid tissue diagnosis method that is suitable for use in the operating room, although further research is required before it can be applied in clinical practice.
随着先进的微创手术技术的发展，并且鉴于功能和美观方面的因素，手术期间对快速准确诊断的需求日益增加。本研究旨在开发一种在简单组织染色后使用共聚焦显微镜的组织诊断方法，该方法无需冷冻和切片。目前，共聚焦显微镜荧光染色尚未普遍用于手术期间的实时诊断。在本文中，我们提出了一种使用Hoechst 33342和伊红的荧光染色方法，该方法无需组织冷冻和切片。尽管在应用于临床实践之前还需要进一步研究，但所提出的方法可作为一种适用于手术室的快速组织诊断方法的一部分。
