Department of Obstetrics and Gynecology, Peking University People's Hospital, Beijing, China.
Key Laboratory of Precision Opto-Mechatronics Technology of Ministry of Education, School of Instrumentation Science & Optoelectronics Engineering, Beihang University, Beijing, China.
J Biophotonics. 2019 Jul;12(7):e201800443. doi: 10.1002/jbio.201800443. Epub 2019 Apr 2.
Platinum chemosensitivity detection plays a vital role during endometrial cancer treatment because chemotherapy responses have profound influences on patient's prognosis. Although several methods can be used to detect drug resistance characteristics, studies on detecting drug sensitivity based on dynamic and quantitative phase imaging of cancer cells are rare. In this study, digital holographic microscopy was applied to distinguish drug-resistant and nondrug-resistant endometrial cancer cells. Based on the reconstructed phase images, temporal evolutions of cell height (CH), cell projected area (CPA) and cell volume were quantitatively measured. The results show that change rates of CH and CPA were significantly different between drug-resistant and nondrug-resistant endometrial cancer cells. Furthermore, the results demonstrate that morphological characteristics have the potential to be utilized to distinguish the drug sensitivity of endometrial cancer cells, and it may provide new perspectives to establish optical methods to detect drug sensitivity and guide chemotherapy in endometrial cancer.
铂类化疗药物敏感性检测在子宫内膜癌治疗中具有重要作用，因为化疗反应对患者的预后有深远影响。尽管有多种方法可用于检测耐药特性，但基于癌细胞动态和定量相位成像来检测药物敏感性的研究却很少。在这项研究中，数字全息显微镜被应用于区分耐药性和非耐药性子宫内膜癌细胞。基于重建的相位图像，定量测量了细胞高度 (CH)、细胞投影面积 (CPA) 和细胞体积的时间演化。结果表明，耐药性和非耐药性子宫内膜癌细胞的 CH 和 CPA 的变化率有显著差异。此外，结果表明形态特征有可能被用于区分子宫内膜癌细胞的药物敏感性，这可能为建立光学方法来检测药物敏感性和指导子宫内膜癌化疗提供新的视角。
