Liu Yongdi, Lin Chaoba, Jia Honghua, Yong Xiaoyu, Xie Xinxin, Wu Xiayuan, Zhou Jun, Wei Ping
College of Biotechnology and Pharmaceutical Engineering, Nanjing Tech University, Nanjing, Jiangsu, People's Republic of China.
Bioenergy Research Institute, Nanjing Tech University, Nanjing, Jiangsu, People's Republic of China.
Environ Technol. 2020 Sep;41(21):2806-2816. doi: 10.1080/09593330.2019.1583290. Epub 2019 Feb 27.
This paper studied the property of three different biofilm carriers added into the anaerobic digestion systems, a granular activated carbon, a polyacrylonitrile, and a polyacrylonitrile modified with diethylenetriamine (PAN-NH). The PAN-NH system kept the maximum biogas and methane production, which were 42.69% and 37.29% higher than the control system, respectively. The value of pH and chemical oxygen demand, the content of total solid and volatile solid, volatile fatty acids concentration, coenzyme F concentration, and microbial community analysis were investigated during the anaerobic digestion process. The PAN-NH system had the highest removal efficiency of the pollutants and regulated the pH of the system better than other systems. The result of high-throughput sequencing analysis showed that the addition of biofilm carriers and mediation with amino-groups adjusted system pH and improved biogas and CH production by reducing the relative abundance of bacteria in the hydrolysis/acidogenesis stages. gradually replaced other methanogens during the experimental runs and was the dominant methanogen at the end of the anaerobic digestion process.
本文研究了添加到厌氧消化系统中的三种不同生物膜载体的性能,分别是颗粒活性炭、聚丙烯腈以及用二亚乙基三胺改性的聚丙烯腈(PAN-NH)。PAN-NH系统的沼气和甲烷产量最高,分别比对照系统高出42.69%和37.29%。在厌氧消化过程中,对pH值、化学需氧量、总固体和挥发性固体含量、挥发性脂肪酸浓度、辅酶F浓度以及微生物群落分析进行了研究。PAN-NH系统对污染物的去除效率最高,并且比其他系统能更好地调节系统的pH值。高通量测序分析结果表明,添加生物膜载体以及氨基介导通过降低水解/产酸阶段细菌的相对丰度来调节系统pH值并提高沼气和CH产量。在实验运行过程中, 逐渐取代了其他产甲烷菌,并在厌氧消化过程结束时成为优势产甲烷菌。