Bai Jinbing, Jhaney Ileen, Daniel Gaea, Watkins Bruner Deborah
Emory University.
Oncol Nurs Forum. 2019 Mar 1;46(2):E48-E59. doi: 10.1188/19.ONF.E48-E59.
To characterize the vaginal microbiome using QIIME 2™ (Quantitative Insights Into Microbial Ecology 2) in women with gynecologic cancer.
SAMPLE & SETTING: 19 women with gynecologic cancer before and after radiation therapy at a comprehensive cancer center in Atlanta, Georgia.
METHODS & VARIABLES: This pilot study analyzed vaginal microbiome communities using a microbiome analysis pipeline, beginning with 16S rRNA gene sequencing and processing through use of a bioinformatics pipeline to downstream microbial statistical analysis.
The findings showed the methods to be robust, and most women with gynecologic cancer showed depletion of Lactobacillus. Compared to those pre-radiation therapy, women post-radiation therapy showed higher abundances of Mobiluncus, Atopobium, and Prevotella but lower abundances of Lactobacillus, Gardnerella, and Peptostreptococcus, which are associated with bacterial vaginosis.
This study presents the fundamentals of human microbiome data collection and analysis methods to inform nursing science. Assessing the vaginal microbiome provides a potential pathway to develop interventions to ameliorate dysbiosis of the vaginal microbiome.
使用QIIME 2™（微生物生态学定量洞察2）对妇科癌症女性的阴道微生物群进行特征分析。
佐治亚州亚特兰大市一家综合癌症中心的19名接受放疗前后的妇科癌症女性。
这项初步研究使用微生物群分析流程分析阴道微生物群群落，从16S rRNA基因测序开始，并通过生物信息学流程进行处理，直至下游的微生物统计分析。
研究结果表明这些方法可靠，大多数妇科癌症女性的乳酸杆菌数量减少。与放疗前相比，放疗后的女性中动弯杆菌属、阿托波菌属和普雷沃菌属的丰度更高，但与细菌性阴道病相关的乳酸杆菌属、加德纳菌属和消化链球菌属的丰度更低。
本研究介绍了人类微生物群数据收集和分析方法的基本原理，为护理科学提供信息。评估阴道微生物群为开发改善阴道微生物群失调的干预措施提供了一条潜在途径。
