Zeber-Lubecka Natalia, Kulecka Maria, Lindner Bogusław, Krynicki Ryszard, Paziewska Agnieszka, Nowakowski Andrzej, Bidzinski Mariusz, Ostrowski Jerzy
Department of Gastroenterology, Hepatology and Clinical Oncology, Centre of Postgraduate Medical Education, Warsaw, Poland.
Department of Genetics, Maria Sklodowska-Curie National Research Institute of Oncology, Warsaw, Poland.
Front Oncol. 2022 Sep 28;12:1005537. doi: 10.3389/fonc.2022.1005537. eCollection 2022.
The cervical microbiome (CM) is a complex ecosystem that can change in response to gynecological cancers. We aimed to evaluate changes in the CM of patients who underwent chemoradiation (CRT) therapy for locally advanced cervical cancer. Before and after CRT, cervical swab samples were collected from 16 patients with squamous cell carcinoma of the cervix, and 30 healthy women. All samples were subjected to 16s rRNA-Seq analysis. In healthy premenopausal women the CM comprised mostly (>90%); the CM community in samples from both pre- and postmenopausal pre-treatment cancer patients was heterogeneous, with a low proportion of in younger cases. On the genus level, 27 and 11 taxa differentiated healthy controls from cancer patients in pre- and postmenopausal age groups, while 31 and 2 genera differentiated pre- and post-radiation samples and pre-radiation and the follow-up samples, respectively. Microbiome diversity was significantly higher in pre-treatment patients than in healthy controls. The results reveal significant alterations in the CM of cervical cancer patients relative to that in healthy controls; these changes were more striking after CRT. However, further research is needed to determine whether alteration of the CM offers new therapeutic options.
宫颈微生物群（CM）是一个复杂的生态系统，会因妇科癌症而发生变化。我们旨在评估接受放化疗（CRT）治疗的局部晚期宫颈癌患者的CM变化。在CRT治疗前后，从16例宫颈鳞状细胞癌患者和30名健康女性中采集宫颈拭子样本。所有样本均进行16s rRNA测序分析。在健康的绝经前女性中，CM主要由（>90%）组成；绝经前和绝经后治疗前癌症患者样本中的CM群落是异质的，在年轻病例中比例较低。在属水平上，27个和11个分类群分别在绝经前和绝经后年龄组中区分健康对照与癌症患者，而31个和2个属分别区分放疗前和放疗后样本以及放疗前和随访样本。治疗前患者的微生物群多样性显著高于健康对照。结果显示，与健康对照相比，宫颈癌患者的CM有显著改变；这些变化在CRT后更为明显。然而，需要进一步研究以确定CM的改变是否提供新的治疗选择。
