Shandong Provincial Key Laboratory of Animal Biotechnology and Disease Control and Prevention, Shandong Agricultural University, 61 Daizong Street, Taian City, Shandong Province 271018, China.
Shandong Provincial Engineering Technology Research Center of Animal Disease Control and Prevention, Shandong Agricultural University, 61 Daizong Street, Taian City, Shandong Province 271018, China.
Poult Sci. 2019 Jul 1;98(7):2772-2780. doi: 10.3382/ps/pez065.
Avian leukosis virus subgroup J has been found to infect many types of chickens with various genetic backgrounds. The ALV-J strain NX0101, which was isolated from broiler breeders in 2001, mainly induces the formation of myeloid cell tumors. However, strain HN10PY01, which was recently isolated from laying hens, mainly induces the formation of myeloid cell tumors and hemangioma. In order to determine the difference in pathogenicity of the 2 strains in broiler chickens, 2 groups of chicken embryos were infected with NA0101 and HN10PY01 separately. A comparison was made of the mortality, oncogenicity, body weights, indexes for immune organs, levels of ALV group-specific antigen p27, and mRNA expression levels of the tumor-related gene, p53, in ALV-J-infected birds and immune organs of theses chickens in response to Newcastle Disease Virus (NDV) and avian influenza virus subtype H9 (AIV-H9) vaccination. The results indicated that strain NX0101 was highly pathogenic in broiler chickens and led to a 30% mortality rate and 45% oncogenicity, compared with the HN10PY01-infected birds. Weight of chickens was also significantly lower after 15 wk (P < 0.05). In addition, the mRNA expression levels of tumor-related p53 in medulla, liver, and lung in broilers infected with strain NX0101 were significantly higher than those infected with strain HN10PY01 (P < 0.05). These results indicated that strain NX0101 had a higher replication ability in broiler chickens. The findings of this study will contribute to further elucidating the mechanisms underlying host susceptibility and tumor classification in ALV-J-infected chickens.
禽白血病病毒亚群 J 已被发现感染多种遗传背景的鸡。2001 年从肉鸡种鸡中分离到的 ALV-J 株 NX0101 主要诱导髓系细胞瘤的形成。然而，最近从蛋鸡中分离到的 HN10PY01 株主要诱导髓系细胞瘤和血管瘤的形成。为了确定 2 株在肉鸡中的致病性差异，用 NX0101 和 HN10PY01 分别感染 2 组鸡胚。比较了 2 组鸡胚死亡率、致癌性、体重、免疫器官指数、ALV 群特异性抗原 p27 水平以及对新城疫病毒（NDV）和 H9 亚型禽流感病毒（AIV-H9）免疫接种后 ALV-J 感染鸡和免疫器官中肿瘤相关基因 p53 的 mRNA 表达水平。结果表明，与 HN10PY01 感染鸡相比，NX0101 株在肉鸡中具有高度致病性，导致 30%的死亡率和 45%的致癌性。15 周后鸡体重也显著降低（P<0.05）。此外，感染 NX0101 株的鸡骨髓、肝脏和肺中肿瘤相关基因 p53 的 mRNA 表达水平明显高于感染 HN10PY01 株的鸡（P<0.05）。这些结果表明 NX0101 株在肉鸡中的复制能力更强。本研究结果将有助于进一步阐明 ALV-J 感染鸡宿主易感性和肿瘤分类的机制。
