College of Agricultural Technology, Shandong Agriculture and Engineering University, Jinan, China; Liaocheng Engineering Technology Research Center for Broiler Healthy Breeding, Liaocheng, China.
College of Agricultural Technology, Shandong Agriculture and Engineering University, Jinan, China.
Poult Sci. 2022 Nov;101(11):102116. doi: 10.1016/j.psj.2022.102116. Epub 2022 Aug 5.
Subgroup K avian leukosis virus (ALV-K) is a new subgroup of avian leukosis virus (ALV) first identified in Chinese indigenous chickens in recent years. In this study, an ALV-K strain was isolated from Luhua chicken in Shandong province, China, and designated SD20LH01. The full-length genomic sequence of SD20LH01 was 7491 bp, which had the highest homology with ALV-K reference strains GDFX0601, GDFX0602 and GDFX0603. The nucleotide homology of env gene of SD20LH01 with reference strains of subgroup A, B, C, D, E, and J was ranged from 57.1 to 93.2%, while 94.1 to 99.4% with other ALV-K reference strains. The nucleotide difference of SD20LH01 mainly clustered with gp85 gene and U3 sequence when compared with the reference strain of ALV-K. In order to investigate the pathogenicity of SD20LH01, SPF chicken embryos were infected by yolk sac inoculation, and 1-day-old chickens were infected by intraperitoneal inoculation of SD20LH01. The results showed that yolk sac inoculation of SD20LH01 could induce persistent viremia, growth retardation and reduce the immune response to NDV and AIV-H9 vaccines. However, intraperitoneal inoculation in 1-day-old chickens could only induce a low level of viremia. In addition, no tumors were found in infected chickens during the animal experiments. This study enriched the genomic sequence data of ALV-K isolated in Chinese indigenous chickens, and laid a foundation for further study on the pathogenesis and prevention of ALV-K.
亚群 K 禽白血病病毒（ALV-K）是近年来在中国本土鸡中首次发现的禽白血病病毒（ALV）的一个新亚群。本研究从中国山东芦花鸡中分离到一株 ALV-K 毒株，命名为 SD20LH01。SD20LH01 的全基因组序列长 7491bp，与 ALV-K 参考株 GDFX0601、GDFX0602 和 GDFX0603 的同源性最高。SD20LH01 的 env 基因与亚群 A、B、C、D、E 和 J 的参考株的核苷酸同源性为 57.1%93.2%，与其他 ALV-K 参考株的同源性为 94.1%99.4%。与 ALV-K 参考株相比，SD20LH01 的核苷酸差异主要集中在 gp85 基因和 U3 序列上。为了研究 SD20LH01 的致病性，本研究通过卵黄囊接种感染 SPF 鸡胚，腹腔接种感染 1 日龄雏鸡。结果表明，SD20LH01 卵黄囊接种可诱导持续性病毒血症、生长迟缓，并降低对 NDV 和 AIV-H9 疫苗的免疫反应。然而，1 日龄雏鸡腹腔接种仅能诱导低水平的病毒血症。此外，在动物实验过程中，感染鸡未发现肿瘤。本研究丰富了中国本土鸡分离的 ALV-K 基因组序列数据，为进一步研究 ALV-K 的发病机制和防治奠定了基础。
