Cell and Cancer Biology Branch, Center for Cancer Research, National Cancer Institute, National Institutes of Health, Bethesda, Maryland.
Laboratory of Pathology, National Cancer Institute, Bethesda, Maryland.
J Histochem Cytochem. 2019 Jun;67(6):453-463. doi: 10.1369/0022155419829050. Epub 2019 Feb 15.
Secretoglobins (SCGBs) are cytokine-like small molecular weight secreted proteins with largely unknown biological functions. Three SCGB proteins, SCGB1A1, SCGB3A1, and SCGB3A2, are predominantly expressed in lung airways. To gain insight into the possible functional relationships among the SCGBs, their protein and mRNA expression patterns were examined in lungs during gestation and in adult mice, using Scgb3a1-null and Scgb3a2-null mice as negative controls, by immunohistochemistry and by qRT-PCR analysis, respectively. The three SCGBs exhibited unique spatiotemporal expression patterns during embryogenesis. The lack of Scgb3a1 or Scgb3a2 did not affect expression of the other Scgb genes as determined by mRNA measurements. Moreover, the lack of Scgb3a1 or Scgb3a2 did not affect development of the pulmonary neuroepithelial bodies during embryogenesis, while the lack of Scgb3a2 may have resulted in slightly fewer ciliated cells than in the wild-type. These results suggest that SCGB1A1, SCGB3A1, and SCGB3A2 each may possess its own unique biological function.
分泌球蛋白（SCGBs）是细胞因子样的小分子分泌蛋白，其生物学功能很大程度上未知。三种 SCGB 蛋白，SCGB1A1、SCGB3A1 和 SCGB3A2，主要在肺气道中表达。为了深入了解 SCGBs 之间可能的功能关系，我们使用 Scgb3a1-/-和 Scgb3a2-/-小鼠作为阴性对照，通过免疫组织化学和 qRT-PCR 分析，分别在妊娠期和成年小鼠的肺部检查了它们的蛋白质和 mRNA 表达模式。这三种 SCGB 在胚胎发生过程中表现出独特的时空表达模式。mRNA 测量结果表明，Scgb3a1 或 Scgb3a2 的缺失并不影响其他 Scgb 基因的表达。此外，Scgb3a1 或 Scgb3a2 的缺失并不影响胚胎发生过程中肺神经上皮小体的发育，而 Scgb3a2 的缺失可能导致纤毛细胞略少于野生型。这些结果表明，SCGB1A1、SCGB3A1 和 SCGB3A2 各自可能具有独特的生物学功能。
