Department of Chemistry and Physics, La Trobe Institute for Molecular Science, La Trobe University, Melbourne, VIC 3086, Australia.
ARC Centre of Excellence in Exciton Science, School of Chemistry, Bio21 Institute, The University of Melbourne, Parkville, VIC 3010, Australia.
Chem Asian J. 2019 Mar 15;14(6):904-909. doi: 10.1002/asia.201900150. Epub 2019 Feb 27.
Collapse of the protein homeostasis (proteostasis) can lead to accumulation and aggregation of unfolded proteins, which has been found to associate with a number of disease conditions including neurodegenerative diseases, diabetes and inflammation. Here we report a maleimide-functionalized tetraphenylethene (TPE)-derivatized fluorescent dye, TPE-NMI, which shows fluorescence turn-on property upon reacting with unfolded proteins in vitro and in live cells under proteostatic stress conditions. The level of unfolded proteins can be measured by flow cytometry and visualized with confocal microscopy.
蛋白质动态平衡（蛋白质稳态）的崩溃会导致未折叠蛋白质的积累和聚集，这与许多疾病状况有关，包括神经退行性疾病、糖尿病和炎症。在这里，我们报告了一种马来酰亚胺功能化的四苯基乙烯（TPE）衍生荧光染料 TPE-NMI，它在体外与未折叠蛋白质反应，并在活细胞中在蛋白质稳态应激条件下表现出荧光开启特性。未折叠蛋白质的水平可以通过流式细胞术进行测量，并通过共聚焦显微镜进行可视化。
