College of Life Sciences, Anhui Normal University, Wuhu 241000, China; Department of Physiology, Wannan Medical College, Wuhu 241000, China.
Department of Physiology, Wannan Medical College, Wuhu 241000, China.
Neurosci Lett. 2019 May 14;701:26-31. doi: 10.1016/j.neulet.2019.02.020. Epub 2019 Feb 12.
The medial prefrontal cortex (mPFC) is implicated in the regulation of drug-seeking behavior, but the specific contributions of the mPFC prelimbic (PL) subdivision and the precise mechanisms underlying heroin abuse remain largely unclear. In the present study, we examined changes in the rhythmic ensemble activity of PL neurons after induction of heroin addiction in rats. Rats were injected daily with saline (control group) or heroin (addiction group) in the light chamber of a light-dark shuttle box, and a video tracking system was used to measure conditioned place preference (CPP) as a sign of addiction. A wireless telemetry system was used to record local field potentials (LFPs) from the PL area during expression of CPP. Before treatment, there was no difference in CPP between groups (P > 0.05). However, rats in the experimental group exhibited significant CPP (P < 0.05) in the light chamber after heroin treatment compared to before treatment and compared to control rats. During CPP, addicted rats demonstrated substantial alterations in relative θ and γ frequency band power (Ps < 0.05); moreover, the θ wave alteration was strongly coupled to γ waves in heat map analyses (P < 0.05). Collectively, these findings implicate heroin-induced alterations in PL area neural activity and θ-γ coupling in heroin addiction.
内侧前额叶皮层（mPFC）参与调节觅药行为，但 mPFC 额前皮质（PL）亚区的具体贡献以及海洛因滥用的精确机制在很大程度上仍不清楚。在本研究中，我们观察了大鼠海洛因成瘾诱导后 PL 神经元节律性集合活动的变化。大鼠在明暗穿梭箱的亮室中每天接受生理盐水（对照组）或海洛因（成瘾组）注射，并使用视频跟踪系统测量条件位置偏好（CPP）作为成瘾的标志。使用无线遥测系统记录 CPP 表达期间 PL 区的局部场电位（LFP）。在治疗前，两组之间的 CPP 没有差异（P>0.05）。然而，与治疗前相比，实验组大鼠在海洛因治疗后在亮室中表现出显著的 CPP（P<0.05），与对照组大鼠相比也是如此。在 CPP 期间，成瘾大鼠表现出相对θ和γ频带功率的显著变化（P<0.05）；此外，热图分析表明θ波的变化与γ波强烈耦合（P<0.05）。总之，这些发现表明海洛因诱导的 PL 区神经活动改变和θ-γ 耦合参与了海洛因成瘾。
